Rubber Elongation Factor (REF), a Major Allergen Component in Hevea brasiliensis Latex Has Amyloid Properties 英文参考文献.docVIP

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Rubber Elongation Factor (REF), a Major Allergen Component in Hevea brasiliensis Latex Has Amyloid Properties 英文参考文献.doc

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RubberElongationFactor(REF),aMajorAllergen ComponentinHeveabrasiliensisLatexHasAmyloid Properties KarineBerthelot1*,SophieLecomte2,YannickEstevez1,Be′ne′dicteCoulary-Salin3,AhmedBentaleb4, ChristopheCullin3,AlainDeffieux1,Fre′de′ricPeruch1* 1CNRS,LCPO,UMR5629,Pessac,France,2CNRS,CBMN,UMR5248,Pessac,France,3Univ.Bordeaux2,CNRS,IBGC,UMR5095,Bordeaux,France,4CNRS,CRPP,UPR8641, Pessac,France Abstract REF (Hevb1) and SRPP (Hevb3) are two major components of Hevea brasiliensis latex, well known for their allergenic properties.Theyareobviouslytakingpartinthebiosynthesisofnaturalrubber,buttheirexactfunctionisstillunclear.They couldbeinvolvedindefense/stressmechanismsaftertappingordirectlyactingontheisoprenoidbiosyntheticpathway. Thestructureofthesetwoproteinsisstillnotdescribed.Inthiswork,itwasdiscoveredthatREFhasamyloidproperties, contrarytoSRPP.WeinvestigatedtheirstructurebyCD,TEM,ATR-FTIRandWAXSandneatlyshowedthepresenceofb- sheetorganizedaggregatesforREF,whereasSRPPmainlyfoldasahelicalprotein.Bothproteinsarehighlyhydrophobicbut differ in their interaction with lipid monolayers used to mimic the monomembrane surrounding the rubber particles. EllipsometryexperimentsshowedthatREFseemstopenetratedeeplyintothemonolayerandSRPPonlybindstothelipid surface. These results could therefore clarify the role of these two paralogous proteins in latex production, either in the coagulationofnaturalrubberorinstress-relatedresponses.Toourknowledge,thisisthefirstreportofanamyloidformed fromaplantprotein.Thissuggestsalsothepresenceoffunctionalamyloidintheplantkingdom. Citation: Berthelot K, Lecomte S, Estevez Y, Coulary-Salin B, Bentaleb A, et al. (2012) Rubber Elongation Factor (REF), a MajorAllergenComponent in Hevea brasiliensisLatexHasAmyloidProperties.PLoSONE7(10):e48065.doi:10.1371/journal.pone.0048065 Editor:AnnaMitraki,UniversityofCrete,Greece ReceivedJuly27,2012;AcceptedSeptember19,2012;PublishedOctober25,2012 Copyright: ? 2012 Berthelot et al. This is an open-access article dis