Screening a Peptide Library by DSC and SAXD Comparison with the Biological Function of the Parent Proteins 英文参考文献.docVIP

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Screening a Peptide Library by DSC and SAXD Comparison with the Biological Function of the Parent Proteins 英文参考文献.doc

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Screening a Peptide Library by DSC and SAXD Comparison with the Biological Function of the Parent Proteins 英文参考文献

ScreeningaPeptideLibrarybyDSCandSAXD: ComparisonwiththeBiologicalFunctionoftheParent Proteins AnaJ.Pe′rez-Berna′1,GeorgePabst2,PeterLaggner2,Jose′ Villala?′n1* 1InstitutodeBiolog?′aMolecularyCelular,Universidad‘‘MiguelHerna′ndez’’, Alicante,Spain,2InstituteofBiophysicsandNanosystemsResearch,AustrianAcademyof Sciences,Graz,Austria Abstract WehaverecentlyidentifiedthemembranotropicregionsofthehepatitisCvirusproteinsE1,E2,coreandp7proteinsby observingtheeffectofprotein-derivedpeptidelibrariesonmodelmembraneintegrity.Wehavestudiedinthisworkthe abilityofselectedsequencesoftheseproteinstomodulatetheLb-LaandLa-HIIphospholipidphasetransitionsaswellas check the viability of using both DSC and SAXD to screen a protein-derived peptide library. We demonstrate that it is feasible to screen a library of peptides corresponding to one or several proteins by both SAXD and DSC. This methodologicalcombinationshouldallowtheidentificationofessentialregionsofmembrane-interactingproteinswhich mightbeimplicatedinthemolecularmechanismofmembranefusionand/orbudding. Citation:Pe′rez-Berna′ AJ,PabstG,LaggnerP,Villala?′nJ(2009)ScreeningaPeptideLibrarybyDSCandSAXD:ComparisonwiththeBiologicalFunctionofthe ParentProteins.PLoSONE4(2):e4356.doi:10.1371/journal.pone.0004356 Editor:RobertJ.Geraghty,UniversityofMinnesota,UnitedStatesofAmerica ReceivedSeptember24,2008;AcceptedDecember9,2008;PublishedFebruary5,2009 Copyright:?2009Pe′rez-Berna′ etal.Thisisanopen-accessarticledistributedunderthetermsoftheCreativeCommonsAttributionLicense,whichpermits unrestricteduse,distribution,andreproductioninanymedium,providedtheoriginalauthorandsourcearecredited. Funding:ThisworkwassupportedbygrantBFU2005-00186-BMC(MinisteriodeCienciayTecnolog?′a,Spain)toJ.V.Thefundershadnoroleinstudydesign,data collectionandanalysis,decisiontopublish,orpreparationofthemanuscript. CompetingInterests:Theauthorshavedeclaredthatnocompetinginterestsexist. *E-mail:jvillalain@umh.es Introduction flaviviruses. This protein is well