Self-Assembled Films of Dendrimers and？Metallophthalocyanines as FET-Based Glucose Biosensors 英文参考文献.docVIP

Sensors 2011, 11, 9442-9449; doi:10.3390/s111009442 OPEN ACCESS sensors ISSN 1424-8220 /journal/sensors Article Self-Assembled Films of Dendrimers and Metallophthalocyanines as FET-Based Glucose Biosensors Nirton C.S. Vieira 1,*, Alessandra Figueiredo 1, Alvaro A.A. de Queiroz 2, Valtencir Zucolotto 1 and Francisco E.G. Guimar?es 1 1 Instituto de Física de S?o Carlos, Universidade de S?o Paulo, CP 369, S?o Carlos, SP 13560-970, Brazil; E-Mails: afigueiredo17@ (A.F.); zuco@ifsc.usp.br (V.Z.); guimaraes@ifsc.usp.br (F.E.G.G) 2 Instituto de Ciências Exatas, Universidade Federal de Itajubá, CP 50, Itajubá, MG 37500-903, Brazil; E-Mail: alencar@.br * Author to whom correspondence should be addressed; E-Mail: nirton@ursa.ifsc.usp.br; Tel.: +55-16-3373-9797; Fax: +55-16-3373-8085. Received: 31 August 2011; in revised form: 20 September 2011 / Accepted: 20 September 2011 / Published: 3 October 2011 Abstract: Separative extended gate field effect transistor (SEGFET) type devices have been used as an ion sensor or biosensor as an alternative to traditional ion sensitive field effect transistors (ISFETs) due to their robustness, ease of fabrication, low cost and possibility of FET isolation from the chemical environment. The layer-by-layer technique allows the combination of different materials with suitable properties for enzyme immobilization on simple platforms such as the extended gate of SEGFET devices enabling the fabrication of biosensors. Here, glucose biosensors based on dendrimers and metallophthalocyanines (MPcs) in the form of layer-by-layer (LbL) films, assembled on indium tin oxide (ITO) as separative extended gate material, has been produced. NH3+ groups in the dendrimer allow electrostatic interactions or covalent bonds with the enzyme (glucose oxidase). Relevant parameters such as optimum pH, buffer concentration and presence of serum bovine albumin (BS