Self-Assembling Complexes of Quantum Dots and scFv Antibodies for Cancer Cell Targeting and Imaging 英文参考文献.docVIP

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Self-Assembling Complexes of Quantum Dots and scFv Antibodies for Cancer Cell Targeting and Imaging 英文参考文献.doc

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Self-Assembling Complexes of Quantum Dots and scFv Antibodies for Cancer Cell Targeting and Imaging 英文参考文献

Self-AssemblingComplexesofQuantumDotsandscFv AntibodiesforCancerCellTargetingandImaging TatianaA.Zdobnova1,2*,OlegA.Stremovskiy1,EkaterinaN.Lebedenko1,SergeyM.Deyev1,3 1InstituteofBioorganicChemistryoftheRussianAcademyofSciences,Moscow,Russia,2DepartmentofBiology,LobachevskyStateUniversityNizhnyNovgorod,Nizhny Novgorod,Russia,3ResearchInstituteofAppliedandFundamentalMedicine,NizhnyNovgorodStateMedicalAcademy,NizhnyNovgorod,Russia Abstract Semiconductorquantumdotsrepresentanovelclassoffluorophoreswithuniquephysicalandchemicalpropertieswhich could enable a remarkable broadening of the current applications of fluorescent imaging and optical diagnostics. Complexes of quantum dots and antibodies are promising visualising agents for fluorescent detection of selective biomarkersoverexpressedintumortissues.Herewedescribetheconstructionofself-assemblingfluorescentcomplexesof quantumdotsandanti-HER1oranti-HER2/neuscFvantibodiesandtheirinteractionswithculturedtumorcells.Abinding strategybasedonaveryspecificnon-covalentinteractionbetweentwoproteins,barnaseandbarstar,wasusedtoconnect quantum dots and the targeting antibodies. Such a strategy allows combining the targeting and visualization functions simplybyvaryingthecorrespondingmodulesofthefluorescentcomplex. Citation: Zdobnova TA,Stremovskiy OA,LebedenkoEN,Deyev SM(2012) Self-Assembling ComplexesofQuantumDots andscFvAntibodiesforCancerCell TargetingandImaging.PLoSONE7(10):e48248.doi:10.1371/journal.pone.0048248 Editor:VladimirV.Kalinichenko,CincinnatiChildren’sHospitalMedicalCenter,UnitedStatesofAmerica ReceivedJuly9,2012;AcceptedSeptember21,2012;PublishedOctober25,2012 Copyright: ? 2012 Zdobnova et al. This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricteduse,distribution,andreproductioninanymedium,providedtheoriginalauthorandsourcearecredited. Funding: Thisresearch wassupportedbytheRussianFoundation forBasic Research(projectnos. 12-04-00757-a,