Sequence homology A poor predictive value for profilins cross-reactivity 英文参考文献.docVIP

Clinical and Molecular Allergy BioMedCentral Research Open Access Sequence homology: A poor predictive value for profilins cross-reactivity Mojtaba Sankian1, Abdolreza Varasteh*1, Nazanin Pazouki1 and Mahmoud Mahmoudi2 Address: 1Immunobiochemistry Lab, Immunology Research Center, Bu-Ali Research Institute, Mashhad, Iran and 2Molecular biology Lab, Immunology Research Center, Bu-Ali Research Institute, Mashhad, Iran Email: Mojtaba Sankian - m_sankian@; Abdolreza Varasteh* - a-varasteh@mums.ac.ir; Nazanin Pazouki - npazouki@; Mahmoud Mahmoudi - Mahmoudi@mums.ac.ir * Corresponding author Published: 10 September 2005 Received: 28 June 2005 Accepted: 10 September 2005 Clinical and Molecular Allergy 2005, 3:13 doi:10.1186/1476-7961-3-13 This article is available from: /1476-7961/3/13 ? 2005 Sankian et al; licensee BioMed Central Ltd. This is an Open Access article distributed under the terms of the Creative Commons Attribution License (/licenses/by/2.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. food allergymelonprofilincross-reactivityepitope Summary Background: Profilins are highly cross-reactive allergens which bind IgE antibodies of almost 20% of plant-allergic patients. This study is aimed at investigating cross-reactivity of melon profilin with other plant profilins and the role of the linear and conformational epitopes in human IgE cross-reactivity. Methods: Seventeen patients with melon allergy were selected based on clinical history and a positive skin prick test to melon extract. Melon profilin has been cloned and expressed in E. coli. The IgE binding and cross-reactivity of the recombinant profilin were measured by ELISA and inhibition ELISA. The amino acid sequence of melon profilin was compared with other profilin sequences. A combination of chemical cleavage and immunoblotting techniques were used to define the role of confo