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Silage produces biofuel for local consumption 英文参考文献
Silageproducesbiofuelforlocalconsumption
Kitamotoetal.
Kitamotoetal.BiotechnologyforBiofuels2011,4:46
/content/4/1/46(1November2011)
Kitamotoetal.BiotechnologyforBiofuels2011,4:46
/content/4/1/46
RESEARCH
OpenAccess
Silageproducesbiofuelforlocalconsumption
HirokoKKitamoto1*?,MitsuoHorita1?,YiminCai2,4,YukikoShinozaki1andKeijiSakaki3
Abstract
Background:Inthenormalprocessofbioethanolproduction,biomassistransportedtointegratedlargefactories
fordegradationtosugar,fermentation,andrecoveryofethanolbydistillation.Biomassnutrientlossoccursduring
preservationanddegradation.Ouraimwastodevelopadecentralizedethanolproductionsystemappropriatefor
farmorco-operativelevelproductionthatusesasolid-statefermentationmethodforproducingbio-ethanolfrom
wholecrops,providescattlefeed,andproducesnowastes.Theideaistoincorporatetraditionalsilagemethods
withsimultaneoussaccharificationandfermentation.Harvested,freshbiomassisensiledwithbiomass-degrading
enzymesandyeast.Multipleparallelreactionsforbiomassdegradationandethanolandlacticacidproductionare
inducedinsolidcultureinhermeticallysealedcontainersataranch.Afterfermentation,ethanoliscollectedonsite
fromthevaporfromheatedfermentedproducts.
Results:Theparallelreactionsofsimultaneoussaccharificationandfermentationwereinducedefficientlyinthe
modelfermentationsystem.Inalaboratory-scalefeasibilitystudyoftheprocess,250goffreshlyharvestedforage
ricewith62%moisturewastreatedwith0.86filterpaperunits/gdrymatter(DM)ofcellulaseand0.32U/gDMof
glucoamylase.After20daysofincubationat28°C,6.4wt.%ofethanolinfreshmatter(equivalentto169g/kgDM)
wasproduced.Whenthe46wt.%moisturewasgatheredasvaporfromthefermentedproduct,74%ofthe
producedethanolwascollected.Organiccellularcontents(suchastheamylaseandpronasedegradablefractions)
weredecreasedby63%andorganiccellwall(fiber)contentby7%comparedtosilagepreparedfromthesame
material.
Conclusions:Weconfirmedthatefficientethanolproductionisinducedinnonsterilizedwholericeplantsina
laboratory-scalesolid-statefermentationsys
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