Silencing of GPNMB by siRNA Inhibits the Formation of Melanosomes in Melanocytes in a MITF-Independent Fashion 英文参考文献.docVIP

下载本文档

6
0
约4.22万字
约 10页
2017-05-13 发布于上海
举报
版权申诉

Silencing of GPNMB by siRNA Inhibits the Formation of Melanosomes in Melanocytes in a MITF-Independent Fashion 英文参考文献.doc

1、原创力文档（book118）网站文档一经付费（服务费），不意味着购买了该文档的版权，仅供个人/单位学习、研究之用，不得用于商业用途，未经授权，严禁复制、发行、汇编、翻译或者网络传播等，侵权必究。。
2、本站所有内容均由合作方或网友上传，本站不对文档的完整性、权威性及其观点立场正确性做任何保证或承诺！文档内容仅供研究参考，付费前请自行鉴别。如您付费，意味着您自己接受本站规则且自行承担风险，本站不退款、不进行额外附加服务；查看《如何避免下载的几个坑》。如果您已付费下载过本站文档，您可以点击这里二次下载。
3、如文档侵犯商业秘密、侵犯著作权、侵犯人身权等，请点击“版权申诉”（推荐），也可以打举报电话：400-050-0827(电话支持时间：9:00-18:30)。
4、该文档为VIP文档，如果想要下载，成为VIP会员后，下载免费。
5、成为VIP后，下载本文档将扣除1次下载权益。下载后，不支持退款、换文档。如有疑问请联系我们。
6、成为VIP后，您将拥有八大权益，权益包括：VIP文档下载权益、阅读免打扰、文档格式转换、高级专利检索、专属身份标志、高级客服、多端互通、版权登记。
7、VIP文档为合作方或网友上传，每下载1次，网站将根据用户上传文档的质量评分、类型等，对文档贡献者给予高额补贴、流量扶持。如果你也想贡献VIP文档。上传文档

SilencingofGPNMBbysiRNAInhibitstheFormationof MelanosomesinMelanocytesinaMITF-Independent Fashion PingZhang1,2.,WeiLiu1,2*.,CanshengZhu3,XiaoyingYuan1,DongguangLi1,WeijieGu1,HuiminMa1, XinXie4,TianwenGao2 1Department ofDermatology,theGeneralHospitaloftheAirForce,Beijing,China,2Department ofDermatology,Xijing Hospital,FourthMilitaryMedicalUniversity, Xi’an,China,3ShaanxiProvincialInstituteforEndemicDiseaseControl,Xi’an,China,4RegLabofTissueEngineering,FacultyofLifeScience,NorthwestUniversity,Xi’an, China Abstract Background:Melanosomesarespecializedmembrane-surroundedorganelles,whichareinvolvedinthesynthesis,storage and transport of melanin. Glycoprotein (transmembrane) non-metastatic melanoma protein b (GPNMB), a melanosome- specific structural protein, shares significant amino acid sequence homology with Pmel-17. Proteomic analysis demonstrated that GPNMB is present in all stages (I-IV) of melanosomes. However, little is known about the role of GPNMBinmelanosomes. Methodology/PrincipalFindings:Usingreal-timequantitativePCR,Westernblottingandimmunofluorescenceanalysis,we demonstrated that the expression of GPNMB in PIG1 melanocytes was up-regulated by ultraviolet B (UVB) radiation. TransmissionelectronmicroscopyanalysisshowedthatthetotalnumberofmelanosomesinPIG1melanocyteswassharply reducedbyGPNMB-siRNAtransfection.Simultaneously,theexpressionlevelsoftyrosinase(Tyr),tyrosinaserelatedprotein1 (Trp1), Pmel17/gp100 and ocular albinism type 1 protein (OA1) were all significantly attenuated. But the expression of microphthalmia-associatedtranscriptionfactor(MITF)wasup-regulated.Intriguingly,inGPNMBsilencedPIG1melanocytes, UVBradiationsharplyreducedMITFexpression. Conclusion: Our present work revealed that the GPNMB was critical for the formation of melanosomes. And GPNMB expressiondown-regulationattenuatedmelanosomeformationinaMITF-independentfashion. Citation: Zhang P,LiuW, Zhu C, Yuan X, LiD,et al. (2012) Silencing of GPNMBby siRNA Inhibits the Formation of