Silencing of XB130 Is Associated with Both the Prognosis and Chemosensitivity of Gastric Cancer 英文参考文献.docVIP

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Silencing of XB130 Is Associated with Both the Prognosis and Chemosensitivity of Gastric Cancer 英文参考文献.doc

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SilencingofXB130IsAssociatedwithBoththePrognosis andChemosensitivityofGastricCancer MinShi1,WeizhenHuang1,LiLin1,DayongZheng1,QiangZuo1,LinWang1,NinaWang1,YajunWu1, YulinLiao2*,WangjunLiao1* 1Department of Oncology, Nanfang Hospital, Southern Medical University, Guangzhou, China, 2Department of Cardiology, Nanfang Hospital, Southern Medical University,Guangzhou,China Abstract XB130 is a newly characterized adaptor protein that was reported to promote thyroid tumor growth, but its role in the progression of other kinds of cancer such as gastric cancer (GC) remains unknown. Accordingly, we investigated the associationbetweenXB130expressionandtheprognosisofGCpatients.Thesubjectswere411patientswithGCinstagesI toIV.XB130expressionwasexaminedinsurgicalspecimensofGC.Kaplan-MeieranalysisandtheCoxproportionalhazards model were used to assess the prognostic significance of XB130 for survival and recurrence. Moreover, GC cells stably transfectedwithXB130shorthairpinRNAwereestablishedtoanalyzetheeffectofXB130onsensitivityofchemotherapy. The results show that both XB130 mRNA and protein expression were detectable in normal gastric tissues. The overall survival time of stage IV patients and the disease-free period after radical resection of GC in stage I–III patients were significantlyshorterwhenimmunohistochemicalstainingforXB130waslowthanwhenstainingwashigh(bothp,0.05). XB130 expression also predicted tumor sensitivity to several chemotherapy agents. Viability of both XB130-silenced SGC7901 cells and wild-type cells was suppressed by 5-fluorouracil (5-FU), cisplatin, and irinotecan in a dose-dependent way,butcisplatinandirinotecanweremoresensitiveagainstsXB130-silencedGCcellsand5-FUshowedhighersensitivity towild-typecells.Whentreatedby5-FU,patientswithhighexpressionofXB130tumorshadahighersurvivalratethan thosewithlowexpressiontumors.ThesefindingsindicatethatreducedXB130proteinexpressionisaprognosticbiomarker forshortersurvivalandahigherrecurrencerateinpatientswithGC,aswellasf