Silencing Quorum Sensing through Extracts of Melicope lunu-ankenda 英文参考文献.docVIP

Sensors 2012, 12, 4339-4351; doi:10.3390/s120404339 OPEN ACCESS sensors ISSN 1424-8220 /journal/sensors Article Silencing Quorum Sensing through Extracts of Melicope lunu-ankenda Li Ying Tan, Wai-Fong Yin and Kok-Gan Chan * Division of Genetics and Molecular Biology, Institute of Biological Sciences, Faculty of Science, University of Malaya, 50603 Kuala Lumpur, Malaysia; E-Mails: etaly87@ (L.Y.T.); yinwaifong@ (W.-F.Y.) * Author to whom correspondence should be addressed; E-Mail: kokgan@.my; Tel.: +603-7967-5162; Fax: +603-7967-4509. Received: 18 January 2012; in revised form: 14 March 2012 / Accepted: 22 March 2012 / Published: 29 March 2012 Abstract: Quorum sensing regulates bacterial virulence determinants, therefore making it an interesting target to attenuate pathogens. In this work, we screened edible, endemic plants in Malaysia for anti-quorum sensing properties. Extracts from Melicope lunu-ankenda (Gaertn.) T. G. Hartley, a Malay garden salad, inhibited response of Chromobacterium violaceum CV026 to N-hexanoylhomoserine lactone, thus interfering with violacein production; reduced bioluminescence expression of E. coli [pSB401], disrupted pyocyanin synthesis, swarming motility and expression of lecA::lux of Pseudomonas aeruginosa PAO1. Although the chemical nature of the anti-QS compounds from M. lunu-ankenda is currently unknown, this study proves that endemic Malaysian plants could serve as leads in the search for anti-quorum sensing compounds. Keywords: anti-quorum sensing; bioluminescence; lecA::lux; Malaysian plants; N-acyl-L-homoserine lactones (AHL); Pseudomonas aeruginosa PAO1; pyocyanin; virulence; swarming motility 1. Introduction Most Gram-negative bacteria use ‘‘quorum sensing’’ (QS) to coordinate their population behaviour including expression of virulence factors through the action of extracellular signal molecules