SLC5A8 Gene, A Transporter of Butyrate A Gut Flora Metabolite, Is Frequently Methylated in African American Colon Adenomas 英文参考文献.docVIP

SLC5A8Gene,ATransporterofButyrate:AGutFlora Metabolite,IsFrequentlyMethylatedinAfrican AmericanColonAdenomas HassanBrim1*,KrishanKumar1,JavadNazarian2,YetribHathout2,AmirJafarian1,EdwardLee ,William 1 Green1,DuaneSmoot1,JongPark3,MehdiNouraie1,HassanAshktorab1* 1 Department of Medicine and Cancer Center, Department of Pathology, Howard University, College of Medicine, Washington, D.C., United States of America,2 Research Center for Genetic Medicine, Children’s National Medical Center, Washington, D.C., United States of America,3 Division of Cancer Prevention and Control, H. Lee Moffitt Cancer Center, Tampa, Florida, United States of America Abstract Background: Colon cancer is one of the leading causes of cancer related deaths. Its impact on African Americans (AAs) is higher than in the general population both in the incidence and mortality from the disease. Colon cancer aggressiveness in AAs as well as non-frequent check-ups and follow up in this population have been proposed as ways to explain the observed discrepancies. These facts made the detection of early carcinogenesis markers in this population a priority. MaterialsandMethods: Here, we analyzed 50 colon adenomas from AA patients for both microsatellite instability (MSI) and the methylation status of SLC5A8 gene. This gene’s product is involved in the transport of butyrate that has anti- proliferative properties through its effects on histone acetylation and gene expression. A proteomic analysis to check the expressed histones in adenoma and normal tissues was also performed. Results: The analyzed samples displayed 82% (n = 41) methylation level of SLC5A8 gene in adenomas. The MSI-H (high) adenoma were about 18% (n = 9) while the rest were mostly MSS (microsatellite stable) with few MSI-L (Low). No association was found between SLC5A8 methylation and the MSI status. Also,