Soluble Immune Complexes Shift the TLR-Induced Cytokine Production of Distinct Polarized Human Macrophage Subsets towards IL-10 英文参考文献.docVIP

下载本文档

2
0
约4.41万字
约 10页
2017-05-13 发布于上海
举报
版权申诉

Soluble Immune Complexes Shift the TLR-Induced Cytokine Production of Distinct Polarized Human Macrophage Subsets towards IL-10 英文参考文献.doc

1、原创力文档（book118）网站文档一经付费（服务费），不意味着购买了该文档的版权，仅供个人/单位学习、研究之用，不得用于商业用途，未经授权，严禁复制、发行、汇编、翻译或者网络传播等，侵权必究。。
2、本站所有内容均由合作方或网友上传，本站不对文档的完整性、权威性及其观点立场正确性做任何保证或承诺！文档内容仅供研究参考，付费前请自行鉴别。如您付费，意味着您自己接受本站规则且自行承担风险，本站不退款、不进行额外附加服务；查看《如何避免下载的几个坑》。如果您已付费下载过本站文档，您可以点击这里二次下载。
3、如文档侵犯商业秘密、侵犯著作权、侵犯人身权等，请点击“版权申诉”（推荐），也可以打举报电话：400-050-0827(电话支持时间：9:00-18:30)。
4、该文档为VIP文档，如果想要下载，成为VIP会员后，下载免费。
5、成为VIP后，下载本文档将扣除1次下载权益。下载后，不支持退款、换文档。如有疑问请联系我们。
6、成为VIP后，您将拥有八大权益，权益包括：VIP文档下载权益、阅读免打扰、文档格式转换、高级专利检索、专属身份标志、高级客服、多端互通、版权登记。
7、VIP文档为合作方或网友上传，每下载1次，网站将根据用户上传文档的质量评分、类型等，对文档贡献者给予高额补贴、流量扶持。如果你也想贡献VIP文档。上传文档

Soluble Immune Complexes Shift the TLR-Induced Cytokine Production of Distinct Polarized Human Macrophage Subsets towards IL-10 英文参考文献

SolubleImmuneComplexesShifttheTLR-Induced CytokineProductionofDistinctPolarizedHuman MacrophageSubsetstowardsIL-10 CarmenA.Ambarus1*,KimC.M.Santegoets2,LennyvanBon2,MarkH.Wenink2,PaulP.Tak1, TimothyR.D.J.Radstake2,DominiqueL.P.Baeten1 1Department of Clinical Immunology and Rheumatology, Academic Medical Center/University of Amsterdam, Amsterdam, The Netherlands, 2Department of Rheumatology,RadboudUniversityNijmegenMedicalCenter,Nijmegen,TheNetherlands Abstract Background: Costimulation of murine macrophages with immune complexes (ICs) and TLR ligands leads to alternative activation. Studies on human myeloid cells, however, indicate that ICs induce an increased pro-inflammatory cytokine production.Thisstudyaimed toclarifytheeffectofICsonthepro-versusanti-inflammatory profileofhumanpolarized macrophages. MaterialsandMethods:MonocytesisolatedfromperipheralbloodofhealthydonorswerepolarizedforfourdayswithIFN- c,IL-4,IL-10,GM-CSF,M-CSF,orLPS,inthepresenceorabsenceofheataggregatedgamma-globulins(HAGGs).Phenotypic polarization markers were measured by flow cytometry. Polarized macrophages were stimulated with HAGGs or immobilizedIgGaloneorincombinationwithTLRligands.TNF,IL-6,IL-10,IL-12,andIL-23weremeasuredbyLuminexand/ orRT-qPCR. Results: HAGGs did not modulate the phenotypic polarization and the cytokine production of macrophages. However, HAGGssignificantlyalteredtheTLR-inducedcytokineproductionofallpolarizedmacrophagesubsets,withtheexceptionof MWIL-4.Inparticular,HAGGs consistently enhancedtheTLR-inducedIL-10production inboth classicallyandalternatively polarizedmacrophages(M1andM2).TheeffectofHAGGsonTNFandIL-6productionwaslesspronouncedanddepended onthepolarizationstatus,whileIL-23p19andIL-12p35expressionwasnotaffected.IncontrastwithHAGGs,immobilized IgGinducedastrongupregulationofnotonlyIL-10,butalsoTNFandIL-6. Conclusion:HAGGsalonedonotalterthephenotypeandcytokineproductionofinvitropolarizedhumanmacrophages.In combination with TLR-ligands, however, HAGGs