Submerged Fermentation of the Edible Mushroom Pleurotus ostreatus in a Batch Stirred Tank Bioreactor as a Promising Alternative for the Effective Production of Bioactive Metabolites 英文参考文献.docVIP

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Submerged Fermentation of the Edible Mushroom Pleurotus ostreatus in a Batch Stirred Tank Bioreactor as a Promising Alternative for the Effective Production of Bioactive Metabolites 英文参考文献.doc

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Submerged Fermentation of the Edible Mushroom Pleurotus ostreatus in a Batch Stirred Tank Bioreactor as a Promising Alternative for the Effective Production of Bioactive Metabolites 英文参考文献

Molecules 2012, 17, 2714-2724; doi:10.3390/moleculeOPEN ACCESS molecules ISSN 1420-3049 /journal/molecules Article Submerged Fermentation of the Edible Mushroom Pleurotus ostreatus in a Batch Stirred Tank Bioreactor as a Promising Alternative for the Effective Production of Bioactive Metabolites Lefki-Maria Papaspyridi 1, Nektarios Aligiannis 2, Evangelos Topakas 1, Paul Christakopoulos 1, Alexandros-Leandros Skaltsounis 2 and Nikolas Fokialakis 2,* 1 BIOtechMASS Unit, Biotechnology Laboratory, School of Chemical Engineering, National Technical University of Athens, 9 Iroon Polytechniou Street, Zografou Campus, GR-15700 Athens, Greece; E-Mails: leriapap@mail.ntua.gr (L.-M.P.); vtopakas@central.ntua.gr (E.T.); hristako@chemeng.ntua.gr (P.C.) 2 Department of Pharmacognosy and Natural Products Chemistry, Faculty of Pharmacy, University of Athens, Panepistimioupolis, GR-15771 Athens, Greece; E-Mails: aligiannis@pharm.uoa.gr (N.A.); skaltsounis@pharm.uoa.gr (A.-L.S.) * Author to whom correspondence should be addressed; E-Mail: fokialakis@pharm.uoa.gr; Tel.: +30-210-7274-727; Fax: +30-210-7274-594. Received: 23 December 2011; in revised form: 20 February 2012 / Accepted: 22 February 2012 / Published: 6 March 2012 Abstract: The aim of this study was to investigate the potential of the submerged fermentation procedure in the production of bioactive metabolites of the common edible mushroom Pleurotus ostreatus. The biomass of the mushroom strain was produced by submerged fermentation in a batch stirred tank bioreactor and extracted by solvents of increasing polarity. The dichloromethane and methanol extract were fractioned by different techniques including Adsorption Chromatography and Fast Centrifugal Partition Chromatography (FCPC). The structures of pure compounds were elucidated with 1D/2D NMR-spectroscopic analyses, and chemical correlations combined with GC/MS and LC/MS experi