Transgenic rice expressing Allium sativum leaf agglutinin (ASAL) exhibits high-level resistance against major sap-sucking pests 英文参考文献.docVIP

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Transgenic rice expressing Allium sativum leaf agglutinin (ASAL) exhibits high-level resistance against major sap-sucking pests 英文参考文献.doc

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Transgenic rice expressing Allium sativum leaf agglutinin (ASAL) exhibits high-level resistance against major sap-sucking pests 英文参考文献

BMC Plant Biology BioMedCentral Research article Open Access Transgenic rice expressing Allium sativum leaf agglutinin (ASAL) exhibits high-level resistance against major sap-sucking pests Bharathi Yarasi1, Vijayakumar Sadumpati1, China Pasalu Immanni2, Dasavantha Reddy Vudem1 and Venkateswara Rao Khareedu*1 Address: 1Centre for Plant Molecular Biology, Osmania University, Hyderabad, 500 007, India and 2Directorate of Rice Research, Rajendranagar, Hyderabad, 500030, India Email: Bharathi Yarasi - bharathi_yerasi@yahoo.co.in; Vijayakumar Sadumpati - vijaycpmb@; China Pasalu Immanni - icpasalu@; Dasavantha Reddy Vudem - vdreddycpmb@; Venkateswara Rao Khareedu* - rao_kv1@ * Corresponding author Published: 14 October 2008 Received: 22 July 2008 Accepted: 14 October 2008 BMC Plant Biology 2008, 8:102 doi:10.1186/1471-2229-8-102 This article is available from: /1471-2229/8/102 ? 2008 Yarasi et al; licensee BioMed Central Ltd. This is an Open Access article distributed under the terms of the Creative Commons Attribution License (/licenses/by/2.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. Abstract Background: Rice (Oryza sativa) productivity is adversely impacted by numerous biotic and abiotic factors. An approximate 52% of the global production of rice is lost annually owing to the damage caused by biotic factors, of which ~21% is attributed to the attack of insect pests. In this paper we report the isolation, cloning and characterization of Allium sativum leaf agglutinin (asal) gene, and its expression in elite indica rice cultivars using Agrobacterium-mediated genetic transformation method. The stable transgenic lines, expressing ASAL, showed explicit resistance against major sap-sucking pests. Results: Allium sativum leaf lectin gene (asal), coding for mannose binding homodimeric protein (ASAL) from garlic plants, has been isolated and in