Verification of the ProPneumo-1 assay for the simultaneous detection of Mycoplasma pneumoniae and Chlamydophila pneumoniae in clinical respiratory specimens 英文参考文献.docVIP

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Verification of the ProPneumo-1 assay for the simultaneous detection of Mycoplasma pneumoniae and Chlamydophila pneumoniae in clinical respiratory specimens 英文参考文献.doc

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Verification of the ProPneumo-1 assay for the simultaneous detection of Mycoplasma pneumoniae and Chlamydophila pneumoniae in clinical respiratory specimens 英文参考文献

Annals of Clinical Microbiology and Antimicrobials BioMedCentral Research Open Access Verification of the ProPneumo-1 assay for the simultaneous detection of Mycoplasma pneumoniae and Chlamydophila pneumoniae in clinical respiratory specimens Rachel R Higgins1, Ernesto Lombos1, Patrick Tang1, Karl Rohoman1, Anne Maki1, Shirley Brown1, Frances Jamieson1,2 and Steven J Drews*1,2,3 Address: 1Ontario Public Health Laboratories, Ministry of Health and Long-Term Care, Toronto, Ontario, Canada, 2Department of Pathobiology and Laboratory Medicine, University of Toronto, Toronto, Ontario, Canada and 3Mount Sinai Hospital, Toronto, Ontario, Canada Email: Rachel R Higgins - rachel.higgins@ontario.ca; Ernesto Lombos - ernesto.lombos@ontario.ca; Patrick Tang - patrick.tang@ontario.ca; Karl Rohoman - karl.rohoman@ontario.ca; Anne Maki - anne.maki@ontario.ca; Shirley Brown - shirley.brown@ontario.ca; Frances Jamieson - frances.jamieson@ontario.ca; Steven J Drews* - steven.drews@ontario.ca * Corresponding author Published: 10 March 2009 Received: 28 November 2008 Accepted: 10 March 2009 Annals of Clinical Microbiology and Antimicrobials 2009, 8:10 doi:10.1186/1476-0711-8-10 This article is available from: /content/8/1/10 ? 2009 Higgins et al; licensee BioMed Central Ltd. This is an Open Access article distributed under the terms of the Creative Commons Attribution License (/licenses/by/2.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. Abstract Background: Mycoplasma pneumoniae and Chlamydophila pneumoniae are major causes of lower and upper respiratory infections that are difficult to diagnose using conventional methods such as culture. The ProPneumo-1 (Prodesse, Waukesha, WI) assay is a commercial multiplex real-time PCR assay for the simultaneous detection of M. pneumoniae and/or C. pneumoniae DNA in clinical respiratory samples. Objective: The aim of this study was t