Preservation of fungi in the teaching laboratory medicine.docVIP

Preservation of fungi in the teaching laboratory medicine [Keywords:] fungi deposited Microbiology Laboratory Medical Mycology, as important biological resources of the natural world, has become an indispensable microbiology experiments the basic materials, applied higher frequencies. This is part of the pathogenic fungus, and how to effectively keep good custody of fungi, the manner in which long-term stability to maintain its biological genetic characteristics, has become our daily work, the problem needs to be examined. 1 Materials and methods 1.1 teaching laboratory strain preserved (1) single-cell fungi: Candida albicans, Cryptococcus neoformans. (2) multi-cellular fungi: Aspergillus niger, Penicillium, Trichoderma. 1.2 Identification of medical fungi strains prior to use should be justified by morphological, biochemical aspects of the identification of qualified before being used. The specific identification method: (1) single-cell fungi: carbon source fermentation tests, producing ascospores and germ tube test, caffeic acid test, urease test; single-cell and multi-cellular fungi identification of common experiment: corn meal Tween 80 agar, a small train observed under the microscope. (2) multi-cellular fungi: Protein hydrolysis test, 37 ℃ growth test, temperature tests, PDA a small culture experiments. 1.3 fungi because of the preservation method used in teaching experimental periods in the number and types of fungi on the different, so suited to their cultivation methods and storage methods not the same. Roughly be divided into regular passage Preservation Law, the two kinds of ultra-low temperature freezing preservation method. 1.3. 1 REGULAR passage Preservation Act (1) liquid medium Preservation Act: single-cell fungi inoculated in RPMI 1640 medium, 37 ℃ cultured 24 h, removed the rear 4 ℃ refrigerator. This method is used to term the recovery of the early low-temperature freezing of bacteria, increasing bacteria cultivation. (2) agar plate medi