Effect of nitrogen-rich cell culture surfaces on type X collagen expression by bovine growth plate chondrocytes.docVIP

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Effect of nitrogen-rich cell culture surfaces on type X collagen expression by bovine growth plate chondrocytes.doc

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Effect of nitrogen-rich cell culture surfaces on type X collagen expression by bovine growth plate chondrocytes

Petitetal.BioMedicalEngineeringOnLine2011,10:4 /content/10/1/4 RESEARCH OpenAccess Effectofnitrogen-richcellculturesurfaceson typeXcollagenexpressionbybovinegrowth platechondrocytes AlainPetit1,2?,CarolineNDemers1?,Pierre-LucGirard-Lauriault3,DorothyStachura1,MichaelRWertheimer3, JohnAntoniou1,2?,FacksonMwale1,2*? *Correspondence:fmwale@ldi.jgh. Abstract mcgill.ca 1LadyDavisInstituteforMedical Research,SMBD-JewishGeneral Hospital,3755ChemindelaCote Background:Recentevidenceindicatesthatosteoarthritis(OA)maybeasystemic diseasesincemesenchymalstemcells(MSCs)fromOApatientsexpresstypeX Ste-Catherine,Montreal,QCH3T 1E2,Canada collagen,amarkeroflatestagechondrocytehypertrophy(associatedwith endochondralossification).WerecentlyshowedthattheexpressionoftypeX collagenwassuppressedwhenMSCsfromOApatientswereculturedonnitrogen (N)-richplasmapolymerlayers,whichwecall“PPE:N”(N-dopedplasma-polymerized ethylene,containingupto36atomicpercentage(at.%)ofN. Methods:Inthepresentstudy,weexaminedtheexpressionoftypeXcollageninfetal bovinegrowthplatechondrocytes(containinghypertrophicchondrocytes)culturedon PPE:N.WealsostudiedtheeffectofPPE:Nontheexpressionofmatrixmoleculessuchas typeIIcollagenandaggrecan,aswellasonproteases(matrixmetalloproteinase-13 (MMP-13)andmoleculesimplicatedincelldivision(cyclinB2).Twootherculture surfaces,“hydrophilic”polystyrene(PS,regularculturedishes)andnitrogen-containing cationpolystyrene(Primaria?),werealsoinvestigatedforcomparison. Results:ResultsshowedthattypeXcollagenmRNAlevelsweresuppressedwhen culturedfor4daysonPPE:N,suggestingthattypeXcollagenisregulatedsimilarlyin hypertrophicchondrocytesandinhumanMSCsfromOApatients.However,the levelsoftypeXcollagenmRNAalmostreturnedtocontrolvalueafter20daysin cultureonthesesurfaces.Cultureonthevarioussurfaceshadnosignificanteffects ontypeIIcollagen,aggrecan,MMP-13,andcyclinB2mRNAlevels. Conclusion:Hypertrophyisdiminishedbyculturinggrowthplatechondrocyteson nitrogen-richsurfaces,amechanismthatisbeneficialforMSCchondro