第9章原核生物的基因调控 .pptVIP

Maintaining Lysogeny Repressor Protein Repressor protein A dimer of 2 identical subunits Each subunit has 2 domains with distinct roles Amino-terminal is the DNA-binding end of molecule Carboxyl-terminal is site of repressor-repressor interaction that makes dimerization and cooperative binding possible Model of Involvement of OL in Repression of PR and PRM Determining the Fate of a l Infection Balance between lysis or lysogeny is delicate Place phage particles on bacterial lawn If lytic infection occurs Progeny spread and infect other cells Circular hole seen in lawn is called plaque Infection 100% lytic gives clear plaque Plaques of l are usually turbid meaning live lysogen is present Some infected cells suffer the lytic cycle, others are lysogenized Battle Between cI and cro Battle Between cI and cro Lysogen Induction When lysogen suffers DNA damage, SOS response is induced Initial event is seen in a coprotease activity in RecA protein Repressors are caused to cut in half, removing them from l operators Lytic cycle is induced Progeny phage can escape potentially lethal damage occurring in host * The ara Control Protein The AraC, ara control protein, acts as both a positive and negative regulator There are 3 binding sites Far upstream site, araO2 araO1 located between -106 and -144 araI is really 2 half-sites araI1 between -56 and -78 araI2 -35 to -51 Each half-site can bind one monomer of AraC The araCBAD Operon The ara operon is also called the araCBAD operon for its 4 genes Three genes, araB, A, and D, encode the arabinose metabolizing enzymes These are transcribed rightward from the promoter araPBAD Other gene, araC Encodes the control protein AraC Transcribed leftward from the araPc promoter Control of the ara Operon In absence of arabinose, no araBAD products needed, AraC exerts negative control Binds to araO2 and araI1 Loops out the DNA in between Represses the operon Control of the ara Operon Presence of arabinose, AraC changes conformation It can no