鱼腥草注射液体外抗菌活性研究-安徽农业科学.PDF

安徽农业科学袁允燥怎则灶葬造 燥枣 粤灶澡怎蚤 粤早则蚤援 杂糟蚤援 圆园园7袁35渊8冤院2264-2265 责任编辑 曹淑华 责任校对 左佳 鱼腥草注射液体外抗菌活性研究 付明，伍贤进 ，刘丹，张俭，魏麟，蒋向辉 （怀化学院生物工程系，湖南怀化 418008 ） 摘要 研究了鱼腥草注射液的体外抗菌活性。以水蒸气蒸馏法制备鱼腥草中药注射液，金黄色葡萄球菌为供试菌种，通过活菌记数 法、加氨苄西林钠 助法对鱼腥草注射液的体外抗菌活性进行研究。结果表明：鱼腥草注射液在体外有一定的抑菌作用，其抗菌 果 比生理盐水明显要强。以活菌记数法、加抗生素 助法测定中草药的体外抗菌活性，方法可行，数据可靠。 关键词 鱼腥草；注射液；抗菌活性 中图分类号 文献标识码 文章编号 （ ） Q949.96 A 0517-6611 2007 08-02264-02 Research on Antibacterial Activity in Vitro of the Houttuynia cordata Thunb Inj ection FU Ming et al (Department of Bioengineering, Huaihua University, Huaihua, Hunan 18008 ） Abstract Antibacterial activity in vitro of the Houttuynia cordata Thunb inj ection was studied in 2004 ~2005. Vapor distillation was used to produce Houttuynia cordata Thunb, a Chinese traditional medicine in inj ection. Antibacterial activity in vitro of the Houttuynia cordata Thunb inj ection was researched by viable count method and ampicillin sodium assistant method using staphylococcus. Result showed that Houttuynia cordata Thunb inj ection had certain antibacterial activity in vitro, which was evidently stronger than normal saline. It was concluded that the viable count method and ampicillin sodium assistant method were feasible and credible to measure antibacterial activity in vitro of Chinese traditional medicine. Key words Houttuynia cordata Thunb; Inj ection; Antibacterial activity 鱼腥草（Houttuynia cordata Thunb）属三白草科（saurura蛳 1.2 方法 cese ）蕺菜属（Houttuynia）野生草本植物，其茎叶搓碎后有鱼 1.2.1 注射液的制备。取鲜鱼腥草样品，由湖南正清制药集 [1-2] 腥味，故名鱼腥草 。鱼腥草分布在长江以南及西藏等省 团按水蒸气蒸馏法制备中草药注射液。 区，尤以四川、湖北、湖南、江苏等省居多。常生长于海拔 1.2.2 菌液的制备。取 37 益条件下培养16 h 的新鲜培养菌