ERK12抑制剂PD98059对大肠癌细胞侵袭和转移的影响.pdfVIP

山东院珩2009 年第49 卷第 34 期 ERKl/2 抑制剂 PD98059 对大肠癌细胞 侵袭和转移的影响 周燕红，刘般飞，主瑶茹 (成?学院医学院，湖北成宁4371(0) 摘要:目的 观察细胞外信号调节蛋白撒酶(丑RK1I2) 抑制剂 PD98059 对大肠癌细胞侵袭和转移的影响，探讨 其可能机制。方法 PD98059 不同浓度(0 、 10、20、40 J.UlloVL )干预大肠癌细胞 24h。用 Boyden 小盒检测其体外 运动和侵袭能力，Westem blot 法检测立RK1I2 磷酸化蛋白和 ERK1!2 ~在白表达情况。结果 随着 PD98059 剂最增 大，大肠庸细胞穿膜数越渐减少(P0.05) ， 磷酸化 ERKl/2 蛋白水平最渐降低(P 0. 05) ， ERK1I2 :m白水平先明 显变化(P0.05) 。结论 PD98059 能抑制大肠癌细胞的i运动和侵袭力;其机制与 PD98059 抑制磷酸化 ERK1!2 有关。 关键词:肠肿瘤;侵袭;转移1 细胞外信号调节策白撒酶jPD98059 中团分类号:民574.6 )l:. 献标志码:A 文章编号:lO02-266X(2ω的34αXl4-02 E仔ect 01 PD98059 on invasion and metastasis of human colon cancer cells ZHOU~ω-hong ,IlU Yi:向，职4NG ~ω{en (Medical College ,Xianning University ， X印刷ing 4371OO ,P. R. China) Abstract: Objective To investigate the effect and mechanism of PD98059 on invasion and metastasis of human colon cancer cells. Methods Colon cancer cells were cultured with different concentration of PD98059 (0μnoνL，lO fU110νL、 20μmνL、40 fU110νL， 8 ERKl/2 specific i刷bitor) for 24 hours. The mobility and invasion ofωlon cancer cells were measured by boyden chamber assay in vitro. The phosphorylation levels of extracellular si伊\al regulated kinase (ERKl/2) were detected by Westem blot method. Results The mobility of LOVO cells was Înhíbited by PD98059 in adose-depended manners. 切\e decreases of tyrosine phosphorylatinn of ERK 1!2 in LOVO were dose dependence of PD98059. ConclusiollS PD98059 could inhibit mobiJjty and invasion of human colon cancer cells.ηle decrease of p-ERK 1I2 may be a possible mechanism. Key words: colon neoplasm; ínvasíon; metastasis; extracelIular signal regulated kinase; PD98059 actín多克隆抗体。 大肠癌转移是一复杂的多步骤过程，寻找大肠 1. 2 实验方法 癌转