Paxillin在高、低转移潜能乳腺癌中的表达及意义.pdfVIP

四川大学学报(医学服〉 2010; 4 1(1) :91 - 94 J Sichuan Univ (Med Sci Edi) , Paxillin 在高、低转移潜能乳腺癌中的表达及意义骨 蔡辉1 ，张涛2 ，汤为学3 ，李少林1 .6. 1. 重庆医科大学基础医学院放射医学教研室〈重庆 400016) I 2. 重庆医科大学附属第一医院肿瘤科〈重庆 400016) I 3. 重庆医科大学基础医学院病理生理学教研室〈重庆 400016) E摘要E 目的 研究 paxil1in 在人商、低转移溶能乳腺癌细胞亚系中的表达以及对肿瘤转移、教附的影响.方 法 以乳腺癌细胞系 MDA-MB-435S 为研究对象，利用细胞穿透人工基底膜能力的差异筛选出高、低转移潜能的 乳腺癌细胞系，利用免疫组化、免疫印迹(Western blot) 、逆转录聚合酶链反应(RT-PCR)检测 paxillin 在商、低转移 潜能乳腺癌细胞中的表达.采用四甲基偶氮嗖蓝(MTT) 法检测细胞毒占附率.结果 免疫组化、Western blot 、 RT­ PCR 检测显示在高转移潜能乳腺癌细胞中 paxillin 在基因和蛋白水平的表达均高于低转移潜能乳腺癌细胞，差异 具有统计学意义(p 均0.01). MTT 法检测高转移潜能乳腺癌细胞的秸附率高于低转移潜能乳腺癌细胞，差异 具有统计学意义(PO.01).结论 Paxil1in 在人乳腺癌转移和毅附过程中发挥重要作用，可能成为抑制肿瘤增殖 和转移的关键性分子. E关键词】 乳腺癌 Paxillin 肿瘤转移 E申图分类号] R737.9 Expression or Paxlllin in Br回st Cancer Cell with High and Low Meta5tatic Potentlality CAI Hui 1 , ZHANG Taoz , TANG Wei-xue3 , LI Sha o-lin 1A • 1. Departmentof Radiation Medicine , Collegeof Basic Medicine , Chongqing Medical University , Chongqing 400016 , China; 2. De户artment of Oncology , the First Affiliated Hospital , Chongqing Medical University , Chongqing 400016 , China ,3. Department of Pathophysiology , College of Basic Medicine , Chongqing Medical University , Chongqing 400016 , China  Corresponding author , E-mail: Shaolinli202@sina. ∞m [Abstract] Objective To study the expression of paxillin in the two subgroups of human breast cancer cells with high and low metastatic potentialities and the effect of paxillin on tumor metastasis and adhesion. Methods Human breast cancer MDA-MB-435s cells were cultured in Transwell chamber with artificial matrigel , two subgroups of MDA-MB-435s cells with different metastatic potentiality were obtained by the ability of cells penetrating artificial matrigel. The expressions