PPARα基因对安卡#215;固始鸡资源群胴体品质的遗传效应分析.pdfVIP

• C T I ABRICULTURAE 华北农学报 .2010.25(6) :14.18 BQREALI-SlnICA PPARα 基因对安卡×固始鸡资源群嗣体品质的 遗传效应分析 田亚东，亢娟娟，孙桂荣，韩瑞丽，康相涛 (河南农业大学牧医工程学院，河南郑州 450002) 摘要:由 793 只安卡 x 固始鸡飞资源群为试验材料，采用 PCR - SSCP 技术和 DNA 测序的方法对 PPARα 基因的 编码区进行 SNP 检测。结果发现，外显子4 存在 1 个多态性位点，经限制性内切酶 BspH 1 酶切后显示出 EE ， EF 、 FF 3 种基因型。利用 SAS8.0 软件分析不同基因型对朋体品质的影响，结果表明 ， PPARα 基因 NC_006088: T3 2311 C 的多态 性显著影响鸡的腹脂重和腹脂率(P 0. 05) ，而对屠体重、皮下脂肪厚、肌间脂肪带宽、半净膛重和全净膛重等无显著 影响( P O. 05 ) 0 EE 型和 EF 型的腹脂重和腹脂率显著高于 FF 型 (P O. 05) ，而 EE 型和 EF 型之间元显著差异 (P 0. 05) 0 PPARα 基因影响脂肪代谢，可以作为分子标记用于鸡脂肪性状的分子标记辅助选择。 关键词:鸡; F 资源群;PPAR α 基因;多态性;嗣体品质 2 申图分类号:S858.03 文献标识码:A 文章编号 :1000 -7091(2010)06 -00t4 -05 Genetic Effects of PPARαGene on Carcass Traits in Anak x Gushi Chicken Resource Population TIAN Ya-dong , KANG Juan-juan , SUN Gui-rong , HAN Rui-li , KANG Xiang-tao (College of Lvestock Husbandry and Veterinary Engineering ,Henan Agricultural University ,Zhengzhou 4筑阻丑，China) Abstract: A Anak x Gush resource population including 793 F2 chickens was founded by F2 design to analyze the PPARαgene polymorphism by the PCR-SSCP combined with sequencing techniques. The single nucleotide poly- morphism (SNP) were found in exon 4 regions of the PPARα. Three kinds of genotypes detected in the resource pop- ulation were defined as EE ,EF and FF by digesting with BspH 1 restriction endonuclease. Effects of different geno- types on carcass traits were evaluated with SAS8.0 software. Results showed that the polymorphism of loci NC 006088:T32311C in PPARαgene was significantly related to the abdominal fat weight ( AFW ) and abdominal fat percentage ( AFP) ( P O. 05 ) , and no significa