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基于ATRChk1P53信号通路探讨三七总皂苷对自然衰老大鼠睾丸生殖细胞DNA损伤的保护作用.pdf
中草药 Chinese Traditional and Herbal Drugs 48 5 2017 3 935
ATR/Chk1/P53
DNA
刘 静,赵海霞,吴秋月,刘珍财,韩贵芳,袁 丁,张长城*
443002
ATR/Chk1/P53 PNSDNA
SPF SD 3 18 PNS 103060 mg/kg
18 10 PNS ig PNS 103060 mg/kg6 d6
HE
DNA γ-H2AXATR Western blotting DNA γ-H2AX
Chk1p-P53P21 PNS
HE PNS
DNA γ-H2AX ATR Chk1
p-P53P21 PNS DNA PNS
DNA ATR/Chk1/P53
DNA ATR/Chk1/P53
R285.5 A 0253 - 2670(2017)05 - 0935 - 06
DOI: 10.7501/j.issn.0253-2670.2017.05.016
Protective effects of Panax notoginseng saponins on testicular DNA damage in
natural aging rats based on ATR/Chk1/P53 signal pathway
LIU Jing, ZHAO Hai-xia, WU Qiu-yue, LIU Zhen-cai, HAN Gui-fang, YUAN Ding, ZHANG Chang-cheng
Medical College of China Three Gorge University, Yichang 443002, China
Abstract: Objective To explore the protective effects of Panax notoginseng saponins (PNS) on testicular DNA damage in natural
aging rats based on ATR/Chk1/P53 signal pathway. Methods SPF SD rats were randomly divided into five groups, with 10 rats in
each group: young control group, aging model group, low, medium, and high dose of PNS-treated group. From 18 months, rats of PNS
low, medium, and high dose groups were received PNS (10, 30, and 60 mg/kg )by stomach lavaging for 6 times per week for 6 months,
respectively. The rats were weighed and euthanized by exsanguination under diethyl ether anesthesia, and testis were immediately
removed, weighed, and the index of testis was calculated. The testicular tissue morphology was observed by HE staining, the
expression and locations of γ-H2AX and ATR were detected by immunohistochemical analysis, and the relative expression levels of
γ-H2AX, Chk1, p-P53, and P21 in testicular tissue were detected by Western blotting. Results Compared with young control group,
the obvious changes of seminiferous tubule were observed in aging model group,
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