基于ATRChk1P53信号通路探讨三七总皂苷对自然衰老大鼠睾丸生殖细胞DNA损伤的保护作用.pdf

中草药 Chinese Traditional and Herbal Drugs 48 5 2017 3 935 ATR/Chk1/P53 DNA 刘 静，赵海霞，吴秋月，刘珍财，韩贵芳，袁 丁，张长城* 443002 ATR/Chk1/P53 PNSDNA SPF SD 3 18 PNS 103060 mg/kg 18 10 PNS ig PNS 103060 mg/kg6 d6 HE DNA γ-H2AXATR Western blotting DNA γ-H2AX Chk1p-P53P21 PNS HE PNS DNA γ-H2AX ATR Chk1 p-P53P21 PNS DNA PNS DNA ATR/Chk1/P53 DNA ATR/Chk1/P53 R285.5 A 0253 - 2670(2017)05 - 0935 - 06 DOI: 10.7501/j.issn.0253-2670.2017.05.016 Protective effects of Panax notoginseng saponins on testicular DNA damage in natural aging rats based on ATR/Chk1/P53 signal pathway LIU Jing, ZHAO Hai-xia, WU Qiu-yue, LIU Zhen-cai, HAN Gui-fang, YUAN Ding, ZHANG Chang-cheng Medical College of China Three Gorge University, Yichang 443002, China Abstract: Objective To explore the protective effects of Panax notoginseng saponins (PNS) on testicular DNA damage in natural aging rats based on ATR/Chk1/P53 signal pathway. Methods SPF SD rats were randomly divided into five groups, with 10 rats in each group: young control group, aging model group, low, medium, and high dose of PNS-treated group. From 18 months, rats of PNS low, medium, and high dose groups were received PNS (10, 30, and 60 mg/kg )by stomach lavaging for 6 times per week for 6 months, respectively. The rats were weighed and euthanized by exsanguination under diethyl ether anesthesia, and testis were immediately removed, weighed, and the index of testis was calculated. The testicular tissue morphology was observed by HE staining, the expression and locations of γ-H2AX and ATR were detected by immunohistochemical analysis, and the relative expression levels of γ-H2AX, Chk1, p-P53, and P21 in testicular tissue were detected by Western blotting. Results Compared with young control group, the obvious changes of seminiferous tubule were observed in aging model group,