高效液相色谱法测定陈皮健胃片中橙皮苷含量.docVIP

高效液相色谱法测定陈皮健胃片中橙皮苷的含量 　　[摘要]目的 建立陈皮健胃片中橙皮苷含量的测定方法。方法 采用高效液相色谱法（HPLC），色谱条件为Inertsil ODS-SP（4.6 mm×150 mm，5 μm）色谱柱分离，检测波长为283 nm，流动相为乙腈-水进行梯度洗脱，流速为1.0 ml/min，柱温为30℃，理论板数≥3000。结果 橙皮苷进样量线性范围为0.10～2.00 μg；在精密度试验、稳定性试验、重复性试验中，橙皮苷峰面积的相对标准偏差（RSD）为2.06%、2.12%、2.14%；橙皮苷的加样回收率为97.36%～102.39%，平均值为99.29 %，RSD为2.06%（n=9）。结论 本法的重现性和专属性良好，加标回收率符合要求，能准确、稳定地对陈皮健胃片中的橙皮苷含量进行定量测定，可作为该制剂的质量控制方法之一 [关键词]陈皮健胃片；橙皮苷；高效液相色谱法 [中图分类号] R927.2 [文献标识码] A [文章编号] 1674-4721（2016）07（b）-0004-04 [Abstract]Objective To establish a method for the determination of hesperidin in Chenpi Jianwei tablet.Methods A high performance liquid chromatography （HPLC） method was established.The chromatographic conditions were as follows：the chromatographic column Inertsil ODS-SP （4.6 mm×150 mm，5 μm） with the mixture of acetonitrile and water as mobile phase in gradient mode，the detection wavelength at 283 nm，the flow velocity of 1.0 ml/min，the column temperature at 30 ℃，the theoretical plate number no less than 3000.Results The linear relationship between content and peak area was obtained during the range of 0.10?C2.00 μg for hesperidin.RSD of the peak area of hesperidin for the precision test，the stability test and the repeatability test was 2.06 %，2.12 % and2.14 % respectively.The recovery rate of hesperidin obtained were in the range of 97.36%?C102.39% and the average of 99.29% with RSD of 2.06% （n=9）.Conclusion This method can be applied to quantitative assay of hesperidin in Chenpi Jianwei tablet with good specificity and reproducibility.The sampling recovery test is in compliance with the requirement，which can be applied to the quality control of Chenpi Jianwei tablet. [Key words]Chenpi Jianwei tablet；Hesperidin；High performance liquid chromatography 陈皮健胃片处方源于第四批全国老中医药专家学术继承工作指导老师，硕士生导师余伯亮主任中医师经验方，由广陈皮、山楂、淮山等组成。该方理气和胃，健脾消食，用于脾胃虚弱、消化不良等症。为便于患者服用，丰富临床用药剂型，研制成陈皮健胃片。研究发现，柑橘属植物中含有丰富的黄酮类化合物，有文献[1]报道，橙皮苷是该植物中重要的活性成分，具有清除氧自由基[2]、抗肿瘤[3]、抗病毒[4]、抗炎[5]、降低血脂、保护心血管[6]等多种功效 橙皮苷含量测定方法有分光光度法[7-8]、荧光光谱法[9]、高效毛细管电泳法[