微波辅助酶法提取绞股蓝皂苷工艺优化.pdf

※工艺技术 食品科学 2016, Vol.37, No.12 1 微波辅助酶法提取绞股蓝皂苷工艺优化 * 张 笛，曾庆梅 ，王 琳，闫春明，高 雅 （合肥工业大学农产品生物化工教育部工程研究中心，安徽 合肥 230009 ） 摘  要：为改善传统水提法提取得率低的问题，研究微波辅助酶法提取绞股蓝皂苷工艺。采用响应面法筛选酶法 提取中复合酶的最佳配比，确定了复合酶最佳配比为果胶酶-半纤维素酶-纤维素酶质量比为4 ∶5 ∶5 ，再利用单因素 试验结合Box-Behnken设计法优化提取工艺。结果表明：影响微波辅助酶法提取绞股蓝皂苷主要因素为复合酶添 加量、酶解温度、酶解时间、微波时间，优化得到的最佳工艺参数为复合酶添加量1.8%、酶解温度52 ℃、酶解时 间2 h 、微波时间4 min ，此工艺条件下绞股蓝皂苷得率为7.88% 。该提取方法与传统水提法相比，产品得率增加了 68%，且提取温度较低，工艺可操作性强。 关键词：微波；复合酶法；响应面法；绞股蓝皂苷 Optimization of Microwave-Assisted Enzymatic Extraction of Gypenosides from Gynostemma pentaphyllum * ZHANG Di, ZENG Qingmei , WANG Lin, YAN Chunming, GAO Ya (Engineering Research Center of Bio-Process, Ministry of Education, Hefei University of Technology, Hefei 230009, China) Abstract: This study addressed the microwave-assisted enzymatic extraction of gypenosides from the whole plants of Gynostemma pentaphyllum as an improvement over the traditional water extraction method, giving a lower extraction yield. Response surface method based on Box-Behnken design was used to optimize the ratio of pectinase to hemicellulase to cellulose for their combined use in the extraction of gypenosides as well as process parameters. The results showed that enzyme dosage, hydrolysis temperature, hydrolysis time and microwave irradiation time were identified as main variables that influence extraction efficiency. The optimum values for these 4 independent variables were 1.8%, 52 ℃, 2 h and 4 min, respectively. The yield of gypenosides was 7.88% under these conditions, which was increased by 68% compared with that obtained with the traditional water extraction method. Due to its low extraction