海水浴场常见致病菌PCR方法和基因芯片检测方法的建立及应用.pdfVIP

摘要 因芯片检验结果与PCR 检测结果100%吻合，说明基因芯片检验方法可靠，结合 其具有高通量的优点，在实际监测工作中可以考虑应用该方法。 本文建立了海水浴场中常见人类致病菌的快速检测方法，该方法可快速筛查 海水中的致病菌，为预防及诊断相关传染病提供可靠的技术手段。其中，海水浴 场中多种致病菌的基因芯片检测方法，在国内尚未见到报道，该检测方法具有灵 敏、特异、快速且有效减少交叉污染概率的优点，是海水浴场中人类致病菌引发 疾病早期诊断的一种新技术，可为评价浴场海水致病菌引发人类健康风险提供技 术支撑。 关键词：致病菌；海水浴场；PCR ；基因芯片 II Abstract Abstract With the rapid growth of the coastal urban population and the development of the coastal economy, the pollution of seawater has drawn people’s attention. Respiratory diseases, skin infections, gastroenteritis diseases caused by pollutional recreational water are common reported at home and abroad. And the conventional indicators such as coliform bacteria have been questioned by many researchers. Therefore, it is necessary to develop a rapid, sensitive and accurate detection method of pathogenic bacteria for reducing the risk of recreation water. During this study, we established PCR methods of fifteen kinds of common pathogenic bacteria of seawater. The pathogenic bacteria include Aeromonas hydrophila, Listeria monocytes , Pseudomonas , Clostridium perfringens, Staphylococcus aureus, Salmonella, Shigella dysentery, Campylobacter jejuni, E.coliO157:H7, Vibrio vulnificus, Streptococcus faecalis, Vibrio cholera (not O1), Vibrio parahaemolyticus, Vibrio alginolyticus, Aeromonas caviae . In addition, we established the methods of microarrays of six of the fifteen bacteria. They are Aeromonas hydrophila , Monocytes listeria , Staphylococcus aureus, Salmonella, E.coliO157:H7, Vibrio alginolyticus. Excep