抗HPV16E6核酶对宫颈癌细胞免疫学特性影响的研究.pdfVIP

·180 · 2001 3 21 2 　Chin J Microbiol Immunol, March 2001, Vol 21, No.2 ·· HPV16E6 郑燕芳　张积仁　屈良鹄　周惠 【】　　HPV16E6 。　 HPV16E6-ribozyme、 CaSKi , CaSKi-R、CaSKi-P 。 3 HLA-1、HLA-2、B7-1B7-2 , CaSKi 。 NK、LAK、CD3AK , CaSKi-R、CaSKi-P、CaSKi 。　CaSKi CaSKi-P HLA-2、B7-1、B7-2 , 。 CaSKi-R HLA-2、B7-1、B7-2 。3 HLA-1 。NK、LAK、CD3AK CaSKi-R CaSKi , CaSKi、CaSKi-R rIL- 2 (CASKI CASKI-R), CaSKi-R CaSKi 。 CaSKi-P CaSKi 。　HPV16E6- ribozymeCaSKi , , 。 【】　;;; The effect of anti-HPV16E6-ribozyme on immune phenotypes of cervical carcinoma cell line　ZHENG Yan- f ang, ZHANGJiren, QULianghu, et al .The Department of Oncology, Zhuj iang Ho pital of the Fir t Military Medical Univer ity , Guangzhou 510282, P .R .China Corresp onding author:ZHENG Yanf ang, E-mail:dr-zheng @163.net 【Abstract】　Objective 　 o study the immune characterization of the cultured cervical cancer cell line transfectedwith anti-HPV16E6-ribozyme, and to explore the possibility of using ribozyme in immune therapy and gene therapy of cervical cancer.Methods　With the method of lipofectin transfection, the anti-HPV16E6-ri- bozyme and empty eucaryotic expressing plasmidswere transfected into CaSKi cell, which are then named as CaS- Ki-R, CaSKi-P respectively.he expression of ribozyme in transfected cellswas observed.he expression of HLA- 1, HLA-2, B7-1, B7-2 genes was studied by flow cytometry.NK, LAK, K lymphocyte stimulated by tumor cells and rIL-2, and CD3AK cellswere induced, and their cytotoxicity were detected in CaSKi-R, CaSKi-P and CaSKi cell.Results　Anti-HPV16E6-ribozyme could be stably expressed in transfected CaSKi cells. he result of flow cytometric analysis showed that anti-HPV16E6-ribozyme could improve the expression of HLA-2, B7-1, B7-2 genes on CaSKi-R cells, but not in the CaSKi-P cell.HLA-1 was highly expressed in all three kinds of cells. Compared wit