嘧菌酯对荔枝霜疫霉病菌不同发育阶段的生物活性.pdfVIP

嚓菌醋对荔枝霜疫霉病菌不同 发育阶段的生物活性 潘汝谦，徐大高，张金妹“ 陈维信2 (华南农业大学，’资源环境学院，2园艺学院，广州 510642 摘要:本文在离体下测定了啼菌醋 (awxys1o01,11)对荔枝霜疫霉病菌 (PeronophythoralachiiChenexKoetal.) 不同发育阶段的生物活性:啥菌醋对荔枝霜疫霉病菌菌丝生长的ECM是。.3212留nli、对抱子囊间接萌发的 EC,值是0.1054pg/ml、对游动抱子游动性的最低抑制浓度 (MIC)是。.025Wm]、对休止抱子萌发的ECM值 是0.011马rg/mlo结果表明，嗜菌酣对荔枝霜疫霉菌不同发育阶段都具有生物活性，同时表现出良好的杀菌 作用和抑菌作用，又以杀菌活性更为强烈。在W40 的浓度下，水杨基氧肘酸 (salicy断dao- 〔acid, SHAM)对荔枝霜疫霉病菌的菌丝生长没有影响;但是SHAM十啥菌酣处理作用于荔枝霜疫霉病菌菌丝生长 的ECn,值是。.1664pg/ml，其P值 (P=E与 (单独杀菌剂)/EC,(杀菌剂十SHAM))是1.93.表明SHAM对 啥菌醋抑制荔枝霜疫霉病菌菌丝生长有增效作用;同时也表明荔枝霜疫霉病菌的旁路氧化酶 (Alternative Oxidase,Aox)是在呼吸传递的主途径受到嗜菌幽阻断后才诱导激活的。 关键词:杀菌剂;荔枝霜疫霉病菌 (Peonophythoralti.W);生物活性;嚓菌酷 TheBiologicalActivitiesofAzoxystrobinonDifferent DevelopmentStagesofPeronophythoralitchii. PanRugiant,XuDagaot,ZhangJinmeit，ChenWe.. (tCollegeofReso二“andEmrironment,2CollegeofHorticultrtreScience, SouthChinaAgriculturalUnivers妙，Guangzhou510642,China.) Absrtact:Thebid嗜calactivitiesofamxystrobinonfw喇developmentofPeronophythomlvchsiiChenexKoeta1.(the causalagentoflycheedownyblight),andthesynergismofSHAMtoaxoxysuobinonthemycelia]growthofP.htchi， testedtotwo.TheECMvalueofxystrobinonnrycelialgrowth,sporangialindirectgermination(场releasing-P-), andcystsporegenninationofP.ltichsiwas0.32121sg/nli,0.1054pg/nd,and0.011911留nd,reaPectively.AndtheMIC (nunimalinhibitoryconcentration)ofazoxystrobinontheinutiliytofwosponsofP.lticlaiwas0.025Wnd.TIwresultsaug gestedthatmox binpossessesbothfungistaticandfungicidelactiviyttoP.Iitchii.SHAM(salicylhydroxarnicacid)had noeffectonntycelialgrowthofP.htauiateoncentrationof40pg/ml,buttheECAvalueofawxystrobinwithSHAM (40pg/rul)tothemycelialgrowthofP.liwhiiwas0.1664Wnli.AridthePvalue(P=ECn(fungicides-SHAM)/ECo (fm心cider+SHAM)). 1.93.ItdemonstratedthatSHAMhadsynergisticeffectontheinhibitionofawxystrobinagainst P.litdui.ItalsoirttpliedthatAl