芜菁NAC转录因子BcNAC2基因的分离及其表达.pdfVIP

园 艺 学 报 2011 ，38 （6 ）：1097 –1103 http: // www. ahs. ac. cn Acta Horticulturae Sinica E-mail: yuanyixuebao@126.com 紫菜薹核基因雄性不育系的创制与利用 * 冯 辉 ，娄广学 （沈阳农业大学园艺学院，辽宁省十字花科蔬菜遗传育种重点实验室，沈阳 110866 ） 摘 要：以复等位基因遗传的白菜核基因雄性不育系00S107 为不育源，采用连续回交转育同时测交 筛选基因型的方法，向紫菜薹自交系 XA500 中转育核不育基因，育成了园艺学性状与XA500 相近，不育株 率和不育度均达到 100%的紫菜薹雄性不育系 GMS500 。用该不育系配成的杂交组合整齐一致，园艺学性 状优异，单位面积产量显著高于对照品种‘紫婷 11 号’。 关键词：紫菜薹；核基因雄性不育系；选育 中图分类号：S 634.6 文献标识码：A 文章编号：0513-353X （2011 ）06-1097-07 Breeding and Utilization of the Genetic Male Sterile Line in Purple Stalk Chinese Cabbage * FENG Hui and LOU Guang-xue （College of Horticulture，Shenyang Agricultural University ，Liaoning Key Laboratory of Genetics and Breeding in Cruciferous Vegetable Crops，Shenyang 110866，China ） Abstract ：In order to solve the problems in the transfer and utilization of genic male sterile line in purple stalk Chinese cabbage ，‘00S107 ’which is a multiple allele inherited genetic male sterile line was used as a source of male sterility in Brassica campestris L. ssp. chinensis var. purpurca Hort. Methods of crossing and backcrossing were applied to transfer the male sterility to a male fertile line ‘XA500 ’. A new genetic male sterile line ‘GMS500 ’with 100% of male sterile plants was obtained ，which was similar to ‘XA500 ’ in horticultural characteristics. An excellent hybridized combination was developed，which is uniform and superior to ‘Ziting 11 ’in yield. Key words ：purple stalk Chinese cabbage ；genetic male sterile line ；breeding 紫菜薹（Brassica campestris L. ssp. chinensis var. purpurca Hort. ）又名红菜薹，是我国湖南、湖 北、四川等长江流域地区普遍栽培的蔬菜作物。紫菜薹两性花，异花授粉，杂种优势十分显著，雄 性不育系的利用是其杂交制种有效途径（汪李平，2005 ）。冯辉（1995）在大白菜中发现了复等位基 因遗传的雄性不育材料，并育成了具有 100%不育株率的大白菜核基因雄性不育系。研究表明，该 不育材料的不育性受同一位点 3 个基因控制：Ms