响应面法优化桑白皮甾醇微波辅助提取工艺.pdfVIP

※王艺技术 20日~ Vo1.34, NO.18 65 自由副事 响应面法优化桑白皮笛醇微波辅助提取王艺 徐艳阳，蔡森森，宋艳锄，李科静 (吉林大学生物与农业工程学院，吉林长春 13∞22) 摘要z 为优化微波辅助提取桑白皮国醇的工艺，分别考察微波功率、微波时间、液固比对桑白皮笛醇得率的影 响:采用响应面法优化微波提取工艺，建立各因素与笛醇得率关系的回归方程。结果表明:最佳工艺条件为微 波功率539W、微波时间41s、液固比如: 1(mL/g) ，此时面醇得率为(7.74 士0.12)mg/g; 实际值与理论值的相对误差 为一4.21% ，二者基本吻合。与溶剂法、超声波法相比，微波辅助法的国醇得率分别提高了1.57%、 19.07% ，提取 时间大大缩短。 关键词:桑白皮:植物面醇:微波辅助提取:响应面:优化 句也因zation ofMicrowave卢Assis时Extraction ofph严osterol from Mulberry R，∞tBarkbyR臼ponseS田face Methodology XU Yan-yang , CAI Sen-sen , SONG Yan-ling , LI Ke才ing (College of Biologica1 and Agricultural Engineering , Jilin University , Changchun 13∞22， China) Abstract: This study was undertak:en to op白血ze the microwave-assisted extraction of phytosterol from the root bark of m且lberry (Morus alba L.) by response surface methodology. The yield of phytosterol was investigated with respect to three variables including microwave output power , irradiation time and solvent-to-solid ratio. As a result , a regression equation was fì出d. The optimal conditions for phytosterol extraction were a solvent-to-solid ratio of 35:1 (mLlg) and microwave irradiation for 41 s with an output power of 539 W. The yield ofphytosterol under the optimized conditions was (7.74 士 0.12) mglg , showing a relative error of -4.21 q毛 compared with the predicted value and increases of 1.57% and 19.07% over those obtained by solvent extraction and ultrasonic-assÎsted ex位action. Mor巳over， the ex国.ction time was considerably shortened by using microwave-assisted extraction in comparison with the other two me由.ods. Key words: mulberry root bark; phytosterol; microwave-assisted extraction; response surface m