响应面法优化那西肽发酵条件.pdfVIP

.430. 中国抗生素杂志2013年6月第38卷第6期 文章编号: 1001-8689(2013)06-0430-04 晌应面法优化那西肤发酵条件 项驷文薛正莲·秦艳飞夏俊 (安徽工程大学生物与化学工程学院，芜湖 241000) 摘要:目的 优化活跃链霉菌NX下NAI0摇瓶发酵生产那西肤的发酵条件。方法 首先用Plackett-Burman设计法筛选出影 响发酵的显著因素，再用响应面法分析优化显著影响因素参数。结果 Plackett-Burman设计法筛选出4个显著影响因素为酵母 粉、黄豆粉、 NaCl和接种量，响应面法优化出它们的最佳数值分别为酵母粉0.31%、黄豆粉6.42%、 NaCIO.41%、接种量5.5%。 结论在此优化条件下，摇瓶效价达到(112 1.28 土26)μglmL， 比优化前提高了10.95%。 关键词:那西肤:活跃链霉菌;发酵条件:响应面优化 中图分类号: R978.1+6 文献标识码:A Optimization of fermentation condition for nosiheptide production by response surface methodology Xiang Si-wen, Xue Zheng-lian, Qin Yan-fei and Xia Jun (Instiωte of Biologic Chemical Engineering ofAnhuì Polytechnic University, Wuhu 241000) Abstract Objective To optimize the fermentation condition of Streptomyces actuosu NXT-NAI0 producing nosiheptide. Methods The Plackett-Burman design and response surface methodology were used to analyse and determine the important factors of the fermentation condition for producing nosiheptide by Streptomyces actuosu NXT-NAI0. Results The yeast power, soybean power, NaCI and inoculum were found to be the important factors and their optimized parameters is yeast power 0.31 %, soybean power 6.42% , NaCl 0.4 1 % and inoculums 5.5% respectively. Conclusions The production ofnosiheptide could reach (1121.28土26)μg/mL by the optimal condition, which was increased by 10.95% compared with the original condition. Key words Nosiheptide; Streptomyces actuosu; Fermentation condition; Response surface of optimization 那西肤[1-2] 又名诺西肤、诺肤菌素，是一种 微生物次级代谢产物产量的提高，主要依赖于 带有5 个唾略环的多肤类抗生素，其分子式为