响应面法优化陶香型窖池中异常威克汉姆酵母发酵产乙酸乙酯条件.pdfVIP

酿酒科技 2016 年第10期（总第268 期）·LIQUOR MAKING SCIENCE TECHNOLOGY 2016 No.10(Tol.268) 7777 DOI：10.13746/j.njkj.2016226 响应面法优化陶香型窖池中异常威克汉姆酵母 发酵产乙酸乙酯条件 侯建光，郭富祥，樊建辉，韩素娜，张俊峰，陈蒙恩 （河南仰韶酒业有限公司博士后研发基地，河南渑池472400 ） 摘 要： 采用中心组合法对陶香型窖池中异常威克汉姆酵母YS/Y/22 发酵产乙酸乙酯条件进行优化。在前期单 因素的基础上，选取发酵后样品中乙酸乙酯含量为响应值，发酵温度、稻壳添加量、发酵时间为自变量，利用Box- Behnken 中心组合法对发酵产乙酸乙酯条件进行优化。结果表明，在发酵温度为28.5 ℃，稻壳添加量为20 % ，时间 为166 h 的最优条件下，乙酸乙酯的产量可达到13.03 g/kg，接近于模型预测值（12.36 g/kg），相较于优化前产量增 长了近2 倍，是正常基酒中的3～5 倍，表明通过中心组合法优化的回归方程具有一定的可行性。 关键词：响应面优化； 异常威克汉姆酵母； 陶香型窖池； 乙酸乙酯 中图分类号：Q93-3 ；TS261.1 ；TS262.3 ；TS261.4 文献标识码：A 文章编号：1001-9286(2016)10-0077-05 OptimizationofEthylAcetate-ProducingConditionsofWickerhamomyces anomalusinTaoxiangPitsbyResponseMethod HOU Jianguang, GUO Fuxiang, FAN Jianhui, HAN Suna, ZHANG Junfeng and CHEN Mengen (Postdoctoral Development Research Base, Yangshao Distillery Co. Ltd., Mianchi, Henan 472400, China) Abstract: The ethyl acetate producing conditions of Wickerhamomyces anomalus in Taoxiang pits were optimized by using response method. On the basis of single factor test, ethyl acetate content in samples after the fermentation used as the response value, fermentation temperature, bran husk adding level, and fermentation time used as the variables, Box Behnken central composite method was adopted to optimize the ethyl acetate producing conditions. The optimum conditions were summed up as follows: fermentation temperature at 28.5 ℃, the adding level of bran husk was 20 %, and fermentation time was 166 h. Under above conditions, the yield of ethyl acetate was 13.03 g/kg, 3 to 5 times of the ethyl acetate content in normal base liquor and close to the predicted value of the model of 12.36 g/kg, and increased almost 2 times than that before the optimization. The study