红千层精油的抗氧化活性及其对中华按蚊幼虫的杀虫活性测定.DOC

红千层精油的抗氧化活性及其对中华按蚊幼虫的杀虫活性测定( ** 重庆师范大学昆虫与分子生物学研究所 重庆 400047 为充分开发利用红千层（Callistemon rigidus R.Br.）植物资源，本研究采用水蒸蒸馏法提取红千层叶的精油（CrEO）测定CrEO的总抗氧化能力丁基羟基茴香醚（BHA）对羟基自由基、1，1-二苯基-2-三硝基苯肼（DPPH）自由基的清除能力并采用浸液法CrEO对中华按蚊幼虫的毒杀。CrEO的总抗氧化活性强于BHT总抗氧化活性CrEO对DPPH自由基的清除率达到50%时的质量浓度(IC50)为6.452 mg/mL，对羟基自由基的IC50值小于1.0×10-5 mg/mL。在毒杀实验中，CrEO对中华按蚊幼虫的LC50（半致死浓度）为293.31 mg/L。研究结果CrEO具有较强的抗氧化活性，对中华按蚊幼虫具有一定的毒杀活性，具备进一步开发成为天然的抗氧化剂和植物源杀虫剂的潜力。 关键词红千层; 精油; 抗氧化能力; 中华按蚊; 杀幼活性 Antioxidant capacity and larvicidal activity of essential oil from Callistemon rigidus* HUANG Ting, RAN Yonghong, LUO Shihui, SI Fengling, YAN Zhentian, CHEN Bin HE Zhengbo** Institute of Entomology and Molecular Biology, College of Life Sciences, Chongqing Normal University, Chongqing 401331, China Abstract In order to fully exploit and utilize the plant resources of Callistemon rigidus, we extracted the essential oil from C. rigidus leaves (CrEO) by steam distillation. The total antioxidant capacity, hydroxyl free radicals and 1,1-diphenyl-2-picrylhydrazyl (DPPH) free radicals scavenging of the CrEO were measured using butylated hydroxytoluene (BHT) and butyl hydroxyanisole (BHA) as positive controls. Meanwhile, the larvicidal activity of CrEO against the larvae of Anopheles sinensis was evaluated by immersion method. The results indicated that CrEO had strong antioxidant ability. The total antioxidant capacity of CrEO was higher than that of BHT but lower than BHA, and its total antioxidant activity increased with concentrations. In addition, CrEO had good free radical scavenging activity. The half maximal inhibitory concentration (IC50) against DPPH radicals was 6.452 mg/mL, and the IC50 value against OH radical was less than 1.0×10-5 mg/mL. Besides that, results of the 24 h mosquito larvicidal assay showed that CrEO had certain toxicity against the larvae of Anopheles sinensis with LC50 value of 293.310 μg/mL. These findings suggested that CrEO has the potential to be developed as a natural antioxidant and botanical mosquit