四环素对胶原生物衍生骨材料细胞相容性的影响.pdfVIP

申国组织工程研究与临床康复 第II 举第35月/j 2007-09-02 出版 Journal of ClinicalRehabili归tive Tissue Engineering Research September 2, 2007 Vol. 11, NO.35 q阻四R 基础 fi 学 四环素对胶原生物衍生骨材料细胞相容性的影响川针 旭 2 ，*1，辛景义 1 兰 梁 Department 01 Orthopaedics and Cytocompatibility of tetracycline loaded collagen bio-derived bone with osteoblasts Traumatology, Tia时in Abstract Hospital, Tianjin 300211 , China; AIM: To investigate the cytocompatibility of collagen bio-derived bone loading tetracycline with osteoblast in rabbits , and Department . 01 analyze the adhesion law in vitro Orthopaedics , General Hospital 01 METHODS: The experiment was carried out in the Laboratory of Tissue Engineering at West China Medical University Lanzhou Military Area from .February 2003 to March 2005. ( Methods: The donor human bone were degreased and deproteinized to form Command 01 Chinese bio-derived bone vector , in which the su巾ce w臼 covered with type- 1 collagen for modification and tetracycline were PLA, Lanzhou 700051 , Gansu absorbed to collagen bio-derived bone. The rabbit osteoblasts were cultured 的 vitro and compounded .to tetracycline Province, China loaded collagen bio-derived bone( Evaluation: 1, 3, 5 and 7 days later, the growth of cells on the surface of compound Liang Jun 食， Doctor, materials was observed under scanning electron microscope , the activity of alkaline phosphatase (ALP) was determined , Associate chiel MTT method was adopted to detect the growth and proliferation of cells , and the cell cycle was tested by flow cytometry physician , Department 01 Orthopaedics and RESULTS: ( The results of scanning electron microscope , ALP activity and MTT assay showed that , the osteoblast