大鼠肝硬化模型制备的改良_向贤宏.pdf

当代医学 2009年12月第15卷第35期 中国介入放射学专题　2009年12月第3卷第6期 大鼠肝硬化模型制备的改良 向贤宏 李家平 陈柳琴 谭国胜 陈伟 杨建勇 李鹤平 [摘要] 目的 对现有肝硬化大鼠造模方法进行改良，构建出稳定的肝硬化大鼠模型并降低制模过程中大鼠的死亡率，为相关的实验提供稳定 可靠的疾病模型。材料与方法 100只Wistar雌性大鼠，按0.5ml／100g剂量予以皮下多点注射50％CCl4 的色拉油溶液，每周注射2次并称量其体重，同 时以10%乙醇溶液为其惟一饮用水源；体重降低大于或等于10g以上时，暂停给药，并改用自来水为饮用水源，直到体重恢复到下降少于10g时继续 上述方案。结果 100只Wistar大鼠14周后，死亡14只，成模86只。结论 经过改良的肝硬化大鼠制模方案可增加建模的成功率，并可形成由慢性肝 炎转化而来的小结节性肝硬化模型。 [关键词] 大鼠；CCl4；酒精；肝硬化 The improvement of hepatic cirrhosis model on rat XIANG Xian-Hong, LI Jia-Ping, CHEN Liu-Qing, TAN Guo-Sheng, CHEN Wei,YANG Jian-Yong, LI He-Ping Department of Interventional Radiology, the First Affiliated Hospital of Sun Yat-sen University, Guangzhou Guangdong 510080, P.R.China 究 研 验 实 与 础 基 [Abstract] Objective To improve the existing model of hepatic cirrhosis on rats,and reduce the mortality rate in modeling process, provide animal models for related experiments. Material and Methods 100 Wistar female rats, CCl4 (analytically pure, density 99.5%), edible white liquor and edible salad oil, gave the hypodermic multi-spots according to the 0.5ml/100g dosage to inject 50% CCl4 salad oil solution, every week injected 2 times and weighed its body weight, simultaneously take 10% alcohol solution as its only source of drinking water;The body weight reduced to10g, suspended giving the medicine, and changed tap water as source of drinking water, restored until the weighing-in after dropping below 10g continued to give the medicine according to the above plan. Results Of 100 Wistar rats with the injection 50% CCl4 +10% ethanol solution, 14 rats died 14 weeks later after injection, all of the remained rats became into models, the achievment rate is 86%.Conclusion The improved p