舒郁散对慢性应激抑郁大鼠行为及脑神经递质的影响.PDF

2009年 5月第 16卷第 5期 中国中医药信息杂志 ·37· 舒郁散对慢性应激抑郁大鼠行为 及脑神经递质的影响 陈利平,王发渭,段冬梅,张媛媛 (中国人民解放军总医院中医科,北京 100853) 摘要：目的 观察舒郁散对慢性应激抑郁大鼠行为及大脑皮质内去甲肾上腺素(NE)、多巴胺(DA)和 5-羟色胺 (5-HT) 含量的影响。方法 50只Wistar大鼠随机分为正常对照组、模型组、阿米替林组、舒郁散小剂量组、舒郁 散大剂量组；对大鼠进行21 d的应激刺激建立慢性应激抑郁大鼠模型,采用Open-Field方法观察各组处理前后2 min 内大鼠行走路线及格子交叉点数的变化,了解大鼠行为的改变,用荧光法测定各组大鼠大脑皮质内NE、DA、5-HT含量 的变化。结果 与正常对照组比较,模型组大鼠水平与垂直运动次数明显少于正常对照组(P ＜0.05),舒郁散大剂量 组、阿米替林组大鼠旷场活动次数明显多于模型组(P ＜0.05)；同时大剂量舒郁散能显著提高慢性应激抑郁模型大 鼠脑内 NE、5-HT 的含量,与模型组比较差异有统计学意义(P ＜0.05)。结论 舒郁散可以改善慢性应激抑郁大鼠行 为,能显著提高慢性应激抑郁大鼠脑内神经递质的含量,上述作用与其剂量呈正相关。 关键词：舒郁散；抑郁症；大鼠行为；神经递质 中图分类号：R285.5 文献标识码：A 文章编号：1005-5304(2009)05-0037-02 Effect of Shuyusan on Behavior and Concentrations of Neurotransmitters in Chronic Stress Depression Rats CHEN Li-ping, WANG Fa-wei, DUAN Dong-mei, et al (Department of TCM, General Hospital of PLA, Beijing 100853, China) Abstract ：Objective To investigate the effect of Shuyusan on the behavior of chronic stress induced depression model rats, as well as concentrations changes of norepinephrine (NE), dopamine (DA) and serotonin (5-HT) in cerebral cortex. Method Fifty mice were randomly divided into 5 groups：normal control group, model group, amitriptyline group, and high/low dose prescription group of Shuyusan. Except normal group, the other groups were given the chronic mild unpredicted stress for 21 days. After 21 days different stimulation, open-Field test was used to estimate the behavior of rats, the concentrations of NE, DA and 5-HT were detected with fluorescence spectrometer method. Results Horizontal and vertical motion of the model group was significantly lower than that o