生物可降解塑料聚β-羟基丁酸酯产生菌的选育.pdfVIP

搜耕 2010 年 39 卷第1 期 珍爱J二等……F吁:物百T 降解锁料聚日，声乎根丁R盘西H 产t荫的~育 寄、: 、乃 部i茹到陆 、、、h 生物可降解塑料聚。经基丁酸醋产生菌的选育. 季爱云，崔志芳，牵春锚，刘 娜 (山东科技大学化学与环境工程工学院，山东，青岛 266510) 摘要;作为一巢可望替代传统塑料的新型可降解生物高分子材料，聚咱要!!:~丁酸葡(PHB) 日益引起人们的重视。 采用尼罗蓝荧光法结合苏丹黑染色法从污水筛选出产 PHB 的细筒，并发瞬提筛得到一株 PHB 产量较高的筒株，PHB -t--I占细胞千爱的 16.64 1岛， PHB 产-1为 0.57 glL。对其进行形态珍养特征和生1重生化特.t:生研究，初步4-定为动胶篱 J乱，编号为办13 以完-13 为出发筒株进行紫外诱变，得到突变筒株 uZ惆13 ， PHB 告~-:I (21. 54 %)和 PHB 产-:1 (0.78 gI川 0 分别比诱突前提高了 29.45 %和 36.84 %，且具有较好的遗传稳定蚀，为微生物法生产生物可降解塑料奠定基础。 关键词:生物可降解塑料:聚。残暴丁峻磁;筛选;紫外诱变;动胶茵 中图分类号:Q93.335 文赋标识码:A 文意编号:1001 -9456(2010)03 -0100 峭的 Breeding of Producing Strains of the Biodegradable Plastic-… Poly -ß-hydroxybutyrate Jl Ai-yun ,CUI Zhi-fang , LI Chun-Iu ,LIU Na (呐le College of Chemical Engineering ,Shandong University of Science and Technology , Qingdao , Shandong 266510 ,China) Abstract: As a new kind of pοlymer material expected to replace the traditional plastics ,poly币-hydroxybutyrate (PHB) was paid great attention in recent years. Strainø producing PHB were screened from the sludge by Nile blue fluorescence combined with the sudan black stain. A strain with higher PHB content was got by secondalγscreening witb 16.64 % PHB content (account for the cell dry weight) and 0.57 glL PHB yield. Colony characteristics ,physiological and biochemical tests showed that the strain could be classified as Zoogloea Itzigohn and then the stain was named as Z-13. Mutation of 由e Z-13 witb UV was studied. After muta事ene恐怕， mutunt uZ-13 was got with PHB content (21. 54 %)