生物破乳剂产生菌XH-1的发酵动力学.pdfVIP

[~，盯 :工莫大学 民旦旦旦L 叩μi JOVRNAL OF JIANGSV VNIVERSITY(Natural Science Edition) doi: 10. 3969/j. issn. 167] -7775.2013.04.016 生物破乳剂产生菌 XH -1 的发酵动力学 侯宁1 ，李大鹏1 ，马放2 ，李春燕1 (1.东北农业大学资源与环境学院，黑龙江哈尔滨 15∞30; 2. 哈尔滨工业大学城市水资源与水环境国家重点试验室，黑龙江哈 尔滨 15∞90) 摘要:为了优化生物破乳剂生产的发酵过程，提高生物破乳剂的产量，对破乳剂产生菌Bacillus TTW- javensis XH - 1 的分批发酵过程进行了研究.根据破乳剂产生菌 XH -1 发酵过程的特点，在Logistic 方程和 Luedeking-Piret 方程的基础上，建立了菌株 XH -1 发酵过程中菌体生长、基质消耗和蛋白 类破乳剂形成的动力学方程.采用 Origin 7.5 软件对试验数据进行处理，得到了破乳菌XH -1 的分 批发酵动力学模型参数.结果表明:模型预测值和试验值吻合较好，所建立的方程能较好地预测其 实际发酵过程，具有很好的适用性，对生物破乳剂的发酵生产具有一定的指导意义. 关键词:破草L 剂产生菌;分批发酵;蛋白类破乳剂;发酵动力学;动力学模型 中图分类号: XI72 文献标志码:A 文章编号: 1671-7775(2013)04 -0455 -05 Fermentation kinetics of bio-demulsifier producing bacteria XH - 1 1 1 Hou Ning , Li Dαipeng , Ma Fα， ni ， Li Chunyαrzl ( 1. College of Resources and Environment , Northeast Agricultural University , Harbin , Heilongjiang 150030 , China; 2. State Key Laboratory of Urban Water Resources and Environment , Harbin Institute of Technology , Harbin , Heilongjiang 15∞90 ， China) Abstract: To optimize the fermentation process of demulsifying bacteria Bacillus 叫avensis XH - 1 , the growth characteristics were investigated to analyze the fermentatÍon kinetics. Based on the characteristics of bacteria XH - 1 , 1ρgistic equation and Luedeking-Piret equation , the kinetic models were established to describe the fermentation features of strain growth , substrate consumption and protein demulsifier ge- neration. The experiment data was processed with Origin 7. 5 software to obtain batch fermentation kine- tics mo