突变株CTM2降解苯酚和4-氯酚的生物降解特性.pdfVIP

Chinese Joumal ofChemical Engíneering , 16(5) 796-800 (2008) Biodegradation of PhenoI and 4-ChIorophenoI by the Mutant Strain CT肌1: 2* JIANG Yan (姜岩)1.2， 3.州，民配N NaQqi (任南琪)2， CAI Xun (蔡掏)1 ， WU Di (吴迪)1 ， QIAO pyan(乔丽艳)1 and LINcSen (林森)1 ~ Department of Chemical Engineeri吨， Daqing Oilfield Engineering Limited Company, Daqing 163712, China ;S巾。1 ofMunicip们nd Environm创al E叩neering， Harbin Instit附ofTechnology， Harbin 15ω90， China School of Lífe Sciences and Chemistry, Harbin University, Harbin 150016, China Abstract 叽le biodegradations of phenol and 4-chlorophenol (4-cp) were studíed using the mutant strain CTM 2 obtained by the He-Ne laser irradiation on wild-type Candida tropicalis. The results showed that the capacity of the CTM 2 to biodegrade 4-cp was increased up to 400 mg.L- within 59 .5 h. ln the dual幡substrate biodegradation , botQ velocity and capacity of the CTM 2 to dcgrade 4-cp increased with low心oflcentration phenol. Atotal of 400 mg.L甲 1 4心p was completely degra~~d within 50.5 h ifl the presence of 300 mgL -I phenol. ηle maximurn 4-cp biodegrada- tion could reach 440 mgL -. with 120 mgL -. pheno l. Low-concentration 4-cp caused great inhibition on the CTM ~to degrade pheno l. In addition, the kinetic behaviors were described using the kinetic model proposed in lhis lab. Keywords biodegradation , phenol , 4心hlorophenol， the mutant strain CTM 2 1 INTRODUCTION capacíty to degrade phenol up to 2000 mg.L -1 [24], and He-Ne laser was then used to irradiate the wild C. Phenol and its derivatives are ubiquitous pol1ut- tropicalis, and consequently the mutant strain CTM 2