促进龙胆紫褪色光度法测定饲料中牛磺酸含量.pdfVIP

20盯川1 回 doi :lO. 7633/j. issn. 1003-6202.2015.04.017 促进龙胆紫褪色光度法测定饲料中牛磺酸含量 李人宇l·2 ，彭 松1 ，陶明涛l ，李咏梅2 ( 1.连云港师范高等专科学校，江苏连云港 222006; 2. 江苏省海洋资源开发研究院，江苏连云港 222005) 摘 要:根据牛磺酸具有促进高棋酸苦甲氧化龙胆紫褪色的原理，考察酸度、高琪酸苦甲用量、龙胆紫用量、温度和时 间对反应的影响，建立分光光度法测定饲料中牛磺酸的新方法。结果表明:最佳反应条件为在 10 ml 比色管中加 入 pH1. 99 盐酸乙酸纳缓冲溶液1. 0 ml 、 2.5 X 10-3 mol/ L 高棋酸匆溶液1. 0 ml 和 2.5 X 10- mol/ L 龙胆紫溶液 1. 0 ml ，室温下反应 11 min。在波长 570 nm 处，吸光度;或小值与牛磺酸质量浓度在 O~O. 25μg/ml 范围内呈良好 的线性关系，表观摩尔吸光系数为 3. 88X 105 L/(mol • cm) ，检出限为1. 91μg/L; 结合起声波提取和离子交换法 预分离技术，采用牛磺酸促进高琪酸何氧化龙胆紫褪色光度法测定饲料中牛磺酸含量，结果与高效液相色谱法一 致，标准偏差为 O. 82%~1. 29%(n=5) ， 回收率为 98. 6%~101. 9% 。 关键词:促进褪色光度法;牛磺酸;高琪酸钟;龙胆紫;饲料;现1J 定 中固分类号:S816. 17;S816. 7 文献标志码:A 文章编号2015)04 一0064 一04 Spectrophotometric determination of taurine content in f，优d using promoting of gentian violet oxidation Li Renyu 1.2 ,Peng Song ,Tao Mingtao , Li Yongmei (1. Lianyungang Normal College , Lianyungang 222006 , China; 2. Jiangsu Marine Resources Development Research Institute , Lianyungang 222005 , China) ABSTRACT: This experiment was conducted to establish a new spectrophotometric method for determination of taurine in feed. Trace taurine has promotive effect on the fading reaction of potassium periodate oxidizing gentian violet. The effect of acidity , potassium periodate and gentian violet dosage , temperature and time on the reaction was studied. The results showed that the optimal reaction conditions were given as follows: in a 10 ml colorimetric tube , 1. 0 ml of hydrochloric acid-sodium acetate buffer solution at pH 1. 99 , 1. 0 ml of 2.5 X 10-3 mo l!L potassium periodate solution and 1. 0 ml of 2.5 X 10- mo l!L gentian violet solution were added. Reaction temperature was room temperature ,