分光光度法测定乳及乳制品中L(-)-羟脯氨酸的改进研究.pdfVIP

伸 ， 中 lLN 陀 · 国 :1 当 吁 J J 阳 口 m 盯 工 盯 业 @l zgrpgy@163.com 分光光度法测定乳及乳制晶中L( 一)一经脯氨酸的改造研究 赵玉窜，周伟同 (黑龙江省质量监督检圳研究院，哈尔滨 100050) 摘 要:针对皮革奶的鉴定长期以来没有正式的国标方法，而句中国检验检疫科学院食品安全所提供的乳与乳制品中动物水解蛋白 鉴定一L(-)- 雍脯氨酸质量分数测定中试样的两种水解方法在实际操作中均有不同程度的缺陷，对试样的水解方法进行了改进，采 用改进后的方法，加标回收率为97.5% ，相对标准偏差为2.25% ，检测限为5 mg/kg，定量F~ 为20 mg/悔，该方法不仅简化了L(-)-发脯氨 酸测定的操作步骤，并且准确可行，具有良好的准确度、精密度、检测限和定量限低等优点。 关键词:皮革奶 ;L( 一)务脯氨酸;分光光度法 中图分类号:TS252.7 文献标识码:A 文章编号: 1001-2230(2013) 10-0036-02 Improvement in determination of L - hydroxyproline hy UV spectrophotomet巧r in milk and dairy products ZHAO Yu一岛， XIAN Wei-yue (The Academe of Quality Inspetion and Researchb for Heilongjiang Province , Haerbin 100050, China) Abstract :There is no fo口nal national standard methods for Leather milk identifìed a long time. Abstract Two hydrolysis methods of samples in Milk and dairy products in the identifìcation of animal proteinhydrolysate-determination ofL(一)-hydro勾rproline， which were published by O伍ce of Food Regulation and providedby National Food Safety Information Center, have defect of different degree in actual experiments. This paper improved the hydrolysís method of samples. With the improved method , the recovery rate of standard addition was 97.5%, the rel- ative standard de飞riation was 2.25% , the límit of detectability was 5 mg/kg and the limit of quantitation was 20 mg/kg. This approach not only simpli且。 the steps of opera出19 in determination of L(一)-hydroxyproline， but also is accurate and feasible. The method have good accuracy, precisíon , low detectabílity and limit of quantiation Key words :Leather milk; L - hydroxyprolíne ;ultravíolet-uisible spectrophotometer 言 o sl