青秦液对高尿酸血症模型大鼠关节免疫性病理损伤的修复作用论文.docVIP

　　青秦液对高尿酸血症模型大鼠关节免疫性病理损伤的修复作用论文 .freelg/kg体重灌服秋水仙碱；青秦液大剂量组给予5 g/(kg·d)的青秦液灌胃,中剂量组给予2.5 g/(kg·d)青秦液灌胃；小剂量组给予1.25 g/(kg·d)灌胃。1周后,在双侧踝关节背侧将尿酸钠溶液注射到关节腔中。72 h后处死动物,.freelent effect of Qingqinye on joint immune pathological injury in hyperuricemia model rats. Methods Rats al group, model group, positive medicine group, Qingqinye high dosage group, Qingqinye middle dosage group and Qingqinye loade butol by gavage. Colchicine 0.09 mg/(kg·d) inistered to the rats of positive medicine group by gavage. Qingqinye high dosage group inistered Qingqinye 5 g/(kg·d), middle dosage group urate ined by ELISA method. Ankle joints alin, decalcified, paraffin embedded and HE Dyed. Pathological section ade to observe the changes of inflammatory cell infiltration. Results IL-8, TNF-α of articular cavity fluid in positive medicine group and Qingqinye group has no significant difference odel group (P＞0.05). But according to the data, IL-8 in Qingqinye loodel group, positive medicine group, Qingqinye high dosage group and Qingqinye middle dosage group. TNF-α in Qingqinye group odel group and positive medicine group, especially in Qingqinye middle dosage group. Pathological section edicine group and Qingqinye group. Conclusion Qingqinye has a certain effect for repairing joint immune pathological injury in hyperuricemia model rats. Key ia；rats injury；immune injury；rat 高尿酸血症是嘌呤代谢障碍、尿酸钠生成增多或排泄减少而导致血尿酸增多的一种代谢性疾病。当血尿酸长期在超饱和浓度以上时,可沉淀成无定型尿酸钠微结晶。由于关节软骨、滑膜及关节周围软组织中血管较少,组织液pH较低,基质中含多糖及结缔组织较丰富,使尿酸容易沉着,引起炎症反应。近年免疫病理学研究表明,高尿酸血症出现急性关节软组织炎症时,白细胞介素(IL)-1、IL-6、IL-8、α-肿瘤坏死因子(TNF-α)表达增强,导致溶酶体、化学介质、毒性自由基等释放,引起组织病理损伤。青秦液是由青蒿、秦艽、秦皮等组成的纯中药制剂,为临床经验方,具有清热解毒、化瘀止痛的功效。本实验采用高尿酸血症急性关节炎大鼠模型,观察青秦液对高尿酸血症急性关节炎大鼠关节免疫性病理损伤的修复作用,探讨青秦液抑制炎性介质过度释放、减少炎症反应过程免疫损伤的作用机理。 1 实验材料 1.1 动物 健康雄性SD大鼠70只,体重(250±10)g；购自北京维通利华实验动物技术有限公司,合格证号SCXK(京)2002-0003。 1.2 受试药物 青秦液组方为：青蒿15 g,秦艽15 g,秦皮10 g,土茯苓15 g,车前子15 g,虎杖15 g,苏木8 g,忍冬藤25 g,黄柏8 g,苍术8 g。上述中药材由北京同仁堂有限公司提供,共煎煮2次。合并2次药液,浓缩成2.2 g原材料/mL,4 ℃储存备用。 1.3 造模药物 乙胺丁醇,杭州民生药业集团有限公司(国药准字：H3302160