益气解毒方乙酸乙酯提取部位对鼻咽癌患者外周血树突状细胞生物学特性的影响.pdfVIP

44 Chin J Otorhinolaryngol Integ Med 2013,Vol.21,No.1 doi:10.3969/j.issn.1007-4856.2013.01.011 益气解毒方乙酸乙酯提取部位对鼻咽癌患者外周血 树突状细胞生物学特性的影响* 1 2 2 2 2 3 胡梅 田道法 何迎春 刘湘 范婧莹 YANG PC 【摘要】目的 察益气解毒方乙酸乙酯提取部位（ethyl acetate extract，EAE）对鼻咽癌（nasopharyngeal carcinoma，NPC）患者外周血树突状细胞（dendritic cells, DCs）生物学特性及其功能的影响。方法 初诊NPC患者10例， 抽取肘静脉血，分离培养外周血单个核细胞（PBMC），用10%胎牛血清RPMI-1640培养基分别加不同浓度益气解毒方EAE 进行体外培养，倒置显微镜下观察DCs的形态学特征，流式细胞仪检测DCs表面特异分子CD1a、CD86和HLA-DR的表达水 平，ELISA法检测培养上清液中IL-12含量。结果 益气解毒方EAE能促进NPC患者PBMC来源的DCs成熟分化并高表达成熟 DCs表面的特异性分子标志物（CD1a，CD86，HLA - -DR），其IL 12分泌水平亦随着培养天数的增加而持续升高。结论 益气 解毒方EAE能明显促进NPC患者外周血来源的DCs成熟分化和功能活性表达。 【关键词】 鼻咽癌；树突状细胞；生物学特性；益气解毒方；乙酸乙酯提取部位 Effects of ethyl acetate extract of Qi-Boosting Toxin-Resolving Formula on the biological properties of induced DCs from the peripheral mononuclear blood cells of untreated NPC patients HU Mei, TIAN Daofa, HE Yingchun, LIU Xiang, FAN Jingying，YANG PC Faculty of Integrative Medicine, Chinese Medicine University of Hunan,Changsha, 410007, China 【Abstract】Objective To investigate the effects of ethyl acetate extract (EAE) from Qi-Boosting Toxin-Resolving Formula (QBTRF) on the biological properties of DCs derived from the peripheral blood mononuclear blood cells (PBMC) of patients with NPC. Methods PBMC were isolated from the peripheral blood of 10 cases with untreated NPC, and cultured in vitro in RPMI-1640 media supplemented With 10% fetal bovine serum, added with different concentrations of EAE extracted from QBTRF. Then, observed were the morphological characteristics of DCs under inverted m