镧示踪法观察常规分割外照射后血脑屏障的超微结构改变.pdfVIP

国际放射医学核医学杂志 2007年11月第31卷第6期 Int J Radiat Med Nucl Med，November 2007，Vol 31，No．6 373 镧示踪法观察常规分割外照射后血脑屏障的超微 结构改变 章龙珍 曹远东 董红燕 于红丽 庄明 【摘要】目的观察Sprague—Dawley(SD)大鼠全脑常规外照射后血脑屏障 (BBB)的超微结 构变化特点及其规律。方法 SD大鼠2O只，分为5个实验组，60co 射线全脑常规分割外照射，各 组受照后16h以硝酸镧为示踪剂，用透射电镜观察BBB超微结构变化。结果 对照组镧颗粒分布在 血管腔内，BBB结构完整；10Gy组少量镧颗粒通过开放的毛细血管内皮细胞间的紧密连接，沉积 于基底膜，但未入脑组织；20Gy组见大部分紧密连接开放，镧颗粒弥漫性分布于神经细胞间隙， 细胞内无镧颗粒沉积；30Gy神经细胞质内见大量、核内见少量镧颗粒沉积；40Gy组部分毛细血管 内皮细胞及神经细胞失去正常形态，结构破坏，大量镧颗粒沉积在细胞核内、外。结论 放射线具 有降低及破坏BBB功能的作用，且与照射剂量呈正相关；30Gy后BBB完全开放，此时进行化疗 为最佳时机；照射40Gy后不易再全脑追加剂量，否则会导致不可逆性放射性脑损伤。 【关键词】血脑屏障；放射疗法；硝酸镧 【中图分类号】R734．2 【文献标识码】A 【文章编号】1673—41 14(2007)06—0373—04 Changes of ultrastructure of blood-brain barrier after whole brain irradiation treatment in rats with lanthanum ZHANG Long-zhen~CA0 Yuan一( n ，D0NG Hong-yan3,YU Hong-li3，ZHUANG Ming4 ( ．Department ofRadiation Oncology,Affiliated Hospital ofXuzhou Medicd Collage，Xuzhou Jangsu 221002,China,．2．Department of Radiation Oncology,Affiliated Hospital Dongnan University,Nanjing Jaltg．5u,210009,China,．3．Laboratory ofResearch Centerfor Neurobiology,Xuzhou Medical Cotle学e，Xuzhou Jangsu 221002，China；4．Experimental Animal Center ofXuzhou Medical College，Xuzhou~iangsu 221002， China) 【Abstract】Objective A pilot study of uhrastructure of blood—brain barrier(BBB)after whole brain irradiation treatment in rats．Methods Twenty Sprague—Dawley rats were devided into 5 groups，To observe the changes of ultrastructure of BBB in 1 6 h after whole brain irradiation with 6~Co~／ray in rats，using lanthanum trace labeling with electron microscopy．Results 1．The lanthanum was only seen in the lumens of capillaries but not in the extravessels to the rats of unirradiation．2．A few tight iunctions of capillaries endothelial cell opened through which the lanthanum entering the outside of capillaries but not in the extravessels after irradiation 10Gy．3．Most tight junctions of endotheli