丹参联合aFGF对新生缺氧缺血性脑损伤大鼠大脑的保护作用.pdfVIP

第25卷第6期 广 东 医 学 院 学 报 Vo1．25 No．6 2007年 12月 JOURNALOFGUANGDONGMEDICAL COLLEGE Dec．2007 611 丹参联合aFGF对新生缺氧缺血性脑损伤大鼠大脑的保护作用 陈志辉 (广东省湛江市妇幼保健院，广东湛江524038) 摘 要：目的 观察丹参联合酸性成纤维细胞生长因子( 、GF)对新生缺氧缺血性脑损伤(HIBD)大鼠大脑的保护作 用，旨在为该病临床药物治疗提供理论依据。方法 制作新生HIBD大鼠模型后给予丹参、aFGF和丹参+aFGF，观察血 清中超氧化物歧化酶(S0D)、一氧化氮(N0)、诱导型一氧化氮合酶(iNOS)的水平变化；计数病变大脑组织中血管内皮生 长因子(vEGF)阳性细胞数、新生血管和脑皮层的凋亡细胞数。结果 (1)空白对照组中NO和iNOS含量明显增多，使用 丹参后NO和iNOS下降，与空白对照组比较差异有统计学意义(P0．01)。(2)正常对照组和空白对照组中新生血管数 和VEGF阳性细胞数少，使用aFGF后两者明显增多，丹参+aFGF组两者数量增多，与丹参组及aFGF组比较差异有统计 学意义(P0．01)。(3)使用丹参或aFGF后脑皮层的细胞凋亡细胞数明显减少，与空白对照组比较有统计学意义(P 0．01)；丹参+aFGF组脑皮层的细胞凋亡细胞数减少更明显，与丹参组、aFGF组比较差异有统计学意义(P0．01)。结论 复方丹参注射液联合aFGF治疗新生HIBD大鼠在保护脑功能、促进血管增生方面有一定的协同作用。 关键词：丹参；酸性成纤维细胞生长因子；缺氧缺血性脑损伤 中图分类号：R 332 文献标识码：A 文章编号：1005—4057(2007)06—0611—03 The protective role of salvia miltiorrhiza plus aFGF in the brain inj ured by the shortage of blood and oxygen in baby rats CHEN Zhi—hui(Maternity and Child Healtheare Hospital of Zhanjiang，Zhanjiang 524038，China) Abstract：Objective To observe the protective role of salvia miltiorrhiza plus in the brain injured by the shortage of blood and oxygen in baby rats．Methods Salvia miltiorrhiza，aFGF or salvia miltiorrhiza+ aFGF were given to the rats with brain injured by the shortage of blood and oxygen，and then serum levels of S0D，NO and iNOS were measured and numbers of VEGF positive cells in brain vessel endothelium，new formed vessels and apoptosis cells in brain were counted．Results (1)The serum levels of NO and iNOS in the salvia miltiorrhiza treated groups were lower than those in the control group，P0．01．(2) The numbers of VEGF positive cells and new form ed vessels were higher in thesalvia miltiorrhiza+aFGF group than those in the salvia miltiorrhiza group， F group or the control groups，P0．01．(3)The numbers