花生属不同区组种质主要植物学性状及分子特性.pdfVIP

2007年 12月 中国油料作物学报 2007，29(4)：402—408 Chinese journal of oil crop sciences 花生属不同区组种质主要植物学性状及分子特性 陈本银，姜慧芳。，廖伯寿，任小平 (中国农业科学院油料作物研究所，湖北武汉 430062) 摘要：从主要植物学性状如主茎粗、主茎高、侧枝长、总分枝数、结果分枝数等22个性状和SSR多态性方面对 花生属不同区组的19份种质资源的主要特性进行了分析。结果表明，不同种质在形态性状方面存在丰富的变异，匍 訇区组的A．rlgonii(G2)、花生区组的A．batizocoi(G8)和A．duranensis(G11)在形态方面具有显著区别于其他种质 的特点。SSR分析结果也证实，不同野生花生种质具有多样化的分子特征，A．rigonii(G2)、A．batizocoi(G8)和A． duranensis(Gl1)等在形态方面具有突出特点的种质，能通过引物检测出来，SSR分析结果与形态性状的分析结果 一 致。引物10D4的检测效率很高，能鉴定19份资源中的l1份。根据25对SSR引物的扩增结果，绘制了19份资源 的指纹图谱。 关键词：花生f植物学性状fsSR；分子特性； 中图分类号：S565．201 文献标识码：A 文章编号：10o7—9084(2007)04--0402—07 Main botanical traits and molecular characters of accessions in Genus Arachis CHEN Ben-yin，JIANG Hui—fang，LIAO Bo—shou，REN Xiao-ping (Oil Crops Research Institute，Chinese Academy of Agricultural Sciences，Wuhan 430062，China) Abstract：Arachis species is an important resource for the improvement of cultivated peanut because of its resistance to many diseases．Since 1979，accessions of wild species of peanut were introduced and conserved at the Oil Crops Research Institute of Chinese Academy of Agricultural Science．It is necessary to investigate the important characters including morphological traits and molecular characters for germplasm utilization．In this study，22 botanical traits including stem thickness，height of main stem， length of branches，total numbers of branches，numbers of branches with pods，etc of 1 9 accessions in 9 species belonging to sections Arachis，Procumbentes and Extranervosae were investigated．The molecular profile of these accessions were analysed through SSR．The results indicated that 19 genotypes could be distinguished by 22 phenotypic traits，and different accessions had different phenotypic traits．A．rigonii of section Proc“rob