蛋白酶体抑制剂MG-132在大鼠重症急性胰腺炎及其肺损伤中的保护作用.pdfVIP

南方医科大学学报(J South Med Univ) ·1845· 蛋白酶体抑制剂MG．132在大鼠重症急性胰腺炎及其肺损伤中的保护作用 李顺ft．，陈 熹，吴 涛，刘继东(西安交通大学医学院第二附属医院普外科，陕西 西安 710004) 摘要：目的观察蛋白酶体抑制剂MG．132在大鼠重症急性胰腺炎(SAP)中的保护作用，并探讨其可能机制。方法SD大 鼠3O只，5％牛磺胆酸钠逆行胆胰管注射制作重症急性胰腺炎(SAP)模型，观察各组大鼠胰腺、肺组织形态学改变，并 测定血清淀粉酶及胰腺、肺组织髓过氧化物酶(MPO)活性。结果MG．132治疗组与胰腺炎组及假手术组比较，血清淀粉 酶、胰腺及肺组织MPO活性下降(PO．05)，组织病理学改变均有不同程度的改善(PO．05)。结论制模前30min腹腔注 射 10mg／kg的MG．132可以明显减轻5％牛磺胆酸钠诱导的大鼠的肺损伤严重程度，并可减轻胰腺炎所致的肺损伤。 关键词：蛋白酶体抑制剂；MG．132；重症急性胰腺炎；肺损伤；髓过氧化物霉 中图分类号：R657．5 文献标识码：A 文章编号：1673-4254(2007)12．1845．03 Protective effect of proteasome inhibitor MG-132 in rats with severe acute pancreatitis and lung illjury LI shun-le，CHEN Xi，WU Tao，LIU Ji—dong Department ofGeneral Surgery,Second Affiliated Hospital of College ofMedicine，Xi’all Jiaotong University，Xi’all 7 10004，China Abstract： Objective To observe the protective effect of the proteasome inhibitor MG一1 32 in rats wim severe acute pancreatitis(sAP)and the associated lung mjury．Methods In rat models ofthe SAP established with mjection of 5％sodium taurocholate into the biliary-pancreatic duct,the changes of the serum amylase and myeloperoxidase 0vn,o)activity in the pancreatic and lung tissues were evaluated．The pathological changes ofthe pancreatic and lung tissues were also observed． Results MG一1 32 significantly decreased serum amylase，pancreatic weight／body weight ratio， and pancreatic and pulmon~ myeloperoxidase activity(PO．05)．Histopathological examinations revealed milder edema，cellular damage，and inflammation inthepancreaticandlungtissuesofratspretreatedwimthepeptide ，O．05)．ConclusionMG一132amelioratesSAP andthe associatedlunginjuryin rats． Keywords：proteasome inhibitor；MG一1 32；severe acute pancreatitis；lung injury；myeloperoxidase 急性重症胰腺炎(SAP)是普外科常见的急腹症 保护作用。 之一，病情凶险，病死率高，死亡原因多由于早期并发 多器官功能衰竭 (MODS)和急性呼吸窘迫综合征 1材料与方法