静态注射化学发光法测定饲料中的粗蛋白质.pdfVIP

安徽农业科学，Journal ofAnhui Agri．Sci．2007，35(35)：11478—11479 责任编辑 金琼琼 责任校对 王森 静态注射化学发光法测定饲料中的粗蛋白质 孟磊·，刘炎超z，任军亮·，谢艳梅· (1．Tqi~农业大学理学院，河南郑州 450002；2．河南农业大学迅捷测试技术有限公司，河南郑州450002) 摘要 【目的1建立测定饲料中粗蛋白质含量的新方法。『方法1采用高灵敏度的化学发光方法，结合有效氮吸收装置，测定饲料中的粗 蛋白质，并与凯氏定氮法比较，进行实际的饲料分析。f结果1静态注射化学发光法选用2 0xl0-4mol／L的Luminol溶液，用量为2．0H0fnl， 待测液的用量为2．0H0 fnl。每100 fnl体系中加入次氯酸钠工作液 1 ml和一定量的硅酸钠。获得最理想发光曲线的仪器工作条件是 一 275 V负高压、100 s发光时间。干扰试验表明0 1 mg／L NH4+干扰允许限为±5％时，1 000倍的cl一、s042．、P0 、NO3-、Ca2~、M 、Fe 、Cu2~ 等离子对测定没有干扰。2种方法测得样品的粗蛋白质含量没有显著差异。『结论1静态注射化学发光法简化了测试方法，缩短了时间。 加标回收率为86．91％～104．60％。RsD5％ =5)，适用于饲料生产部门的质量控制。 关键词 化学发光；饲料；粗蛋白；NH4+ 中图分类号 S816 文献标识码 A 文章编号 o517—6611(2007)35—11478—02 Detection on Crude Protein in Feed by Static Injection MENG Lei et al fCollege of Science，Henan A cultural University，Zhengzhou，Henan 45O002) Abstract [Objective】111e research aimed to establishment the new method of detecting crude protein in feed．【Method】111e crude protein in feed was detected by using chemiluminescence method with high sensitivity combined with device of effective nitrogen absorption and its detection result was compared with the result by micro-kjeldahl method for practical feed analysis．『Result1 In static chemiluminescence me山od， Luminol solution of 2．OxlO~mol／L was chose with the dosage of 2．00 ml and the dosage of ready solution was also 2．OO m1．The working condition of instrument that could obtain most idea luminescence CHIVe was negative high pressure of一275 V and luminescence times of 100 S．The interfering test showed that when the permitting limit of interference at 0．1 mg／L NH4 was±5％，the ions such as CI-,SO4~-，P043．-，NO3-,ca2 ，Mg2+， Fe3+and Cu2~of 1 000 fold had no interference．The crude protein content in samples detected by 2 kinds of methods had no significant difference ． 『Conclusion1 The