黄芩苷对小鼠T、B淋巴细胞增殖的影响.pdfVIP

江西医学院学报 2007年第47卷第6期 ActaAcademiae Medicinae Jiangxi 2007，Vol 47，N06 黄芩苷对小鼠T、B淋巴细胞增殖的影响 王 敏 ，刘旭平。，张建勤 ，姜青龙 ，孙 午 ，傅颖媛 (1．南昌大学医学院免疫学教研室，南昌330006；2．南昌大学抚州医学分院病原学教研室，江西抚州344000； 3．万载县人民医院放射科，江西 万载 336100；4．江西省仁丰药业质保部，江西抚州344000) 摘要：目的 探讨黄芩苷对小鼠T、B淋巴细胞增殖的影响。方法 用尼龙棉柱分离T、B淋巴细胞，MTT比色法 检测不同浓度黄芩苷单独作用及其与丝裂原(ConA，LPS)共同作用对小鼠T、B淋巴细胞增殖作用的影响。结果 0．25～2 mg／mL的黄芩苷显著促进T淋巴细胞增殖(PO．01)，0．125～2 mg／mL的黄芩苷显著增强ConA诱导 的T淋巴细胞增殖作用(PO．01)。0．25～2 mg／mL的黄芩苷显著促进B淋巴细胞增殖(PO．05或PO．01)， 且显著增强LPS诱导的B淋巴细胞增殖作用(PO．05或 PO．01)。结论 黄芩苷显著促进小鼠T、B淋巴细胞 增殖，并与ConA、LPS对T、B淋巴细胞有协同刺激作用。 关键词：黄芩苷；T淋巴细胞；B淋巴细胞；小鼠 中图分类号：R392．12 文献标识码：A 文章编号：10Oo一2294(2007)06—0017～o3 The Effect of Baicalin on the Proliferation 0f T or B Lymphocyte in Mice WANG Min 一，LIU Xu-ping。，ZHANG Jian—qin ，JIANG Qing-long ，SUN Wu ，FU Ying-yuan (1．Department of Immunity，Medical College of Nanchang University，Nanchang 330006，China； 2．Department of Pathogeny，Fuzhou Medical College of Nanchang University，Fuzhou 344000，China； 3．Department of R0 gg 0￡0g ，the People’S Hospital of Wanzai，Wanzai 336100，China； 4．Department of Quality，Jiangxi Renfeng Medical Company，Fuzhou 344000，China) ABSTRACT：Objective To investigate the effect of baicalin on the proliferation of T or B lym— phocytes in Mice．Methods The lymphocytes including T and B lymphoytes were separated by nylon wool cotton column．The effect of baicalin on the proliferation of T or B lymphocytes in mice were tested by MTT assay．Results Baicalin could significantly promote the proliferation of T lymphocytes at the concentration of 0．25，0．5，1，2 mg／mI (P0．O1)and enhance the effect of ConA on the proliferation of T lymphocytes at the concentration of 0．125，0．25，0．5，1，2 mg／mI (P％O．O1)．Baicalin could significantly promote the proliferation of B lymphocytes and increased the stimulation indi