As2O3对人胃癌BGC-823细胞端粒酶的影响.pdfVIP

肿瘤防治研究2007年第34卷第12期 ·927· As2O3对人胃癌BGC一823细胞端粒酶的影响 梅树江 ，尹家俊 ，王晓梅 Effects of Arsenic Trioxide(AS2 03)on Telomerase Activity of Human Gastric Cancer BGC- 823 Cell Line MEI Shu-jiang ，YIN Jia_jun2，WANG Xiao-mei 1．ShenzhenMunicipal Center for Disease Control and Prevention，Shenzhen 518020，China；2．Affil— iated Zhongshan Hospital of Dalian Universit；3．Institute 0_厂L fe Science，Shenzhen University Corresponding Author：ⅥN Jia—jun，E-mail~．jiajunyin1 106 yahoo．com．cn Abstract：Objective To evaluate telomerase activity of human gastric cancer cell Line BGC-823 cultured with different concentration of Arsenic Trioxide and genetic transcription level of human telomerase re— verse transcriptase(hTERT)．Methods The cell activity of BGC-823 cells was measured using M下r method after BGC一823 cells cultured with various concentrations of As2 03．Changes in apoptosis of I~GC- 823 cells were analyzed with flow cytometry(FCM)．Telomerase activity was determined by telomeric repeat amplification protocol PCR ELISA(TRAP PCR ELISA)．The mRNA level of hTERT gene was tested by semi-quantitive RT—PCR Results The proliferation of BGC一823 cells cultured with A82 03 was significantly inhibited and result in cells apoptosis．The tendency of proliferation inhibition depended on As2 03 dose increasing and administrated time prolong．Meanwhile，telomerase activities were markedly decreased and genetic transcription expressions of hTERT were obviously suppressed．These changes al— SO show obvious time-Dose-response relationship．Conclusion As2()3 could inhibit the generation of EGG- 823 cells and activation of telomerase．It maybe a good telomerase inhibitor，this is value for clinical appli— cation of tumor therapy． Key words：Arsenic Trioxide；[~JC-823 Cell Line；Telomerase；Human telomerase reverse transcriptase 摘 要：目的 观察三氧化二砷(As 03)对人胃癌BGC-823细胞端粒酶活性及端粒酶逆转录酶基因转 录的影响。方法 用不同浓度的三氧化二砷作用于人胃癌BGC-823细胞，通过MTT法测定细胞活力； 流式细胞术检测细胞