糖化酶降解壳聚糖的研究论文.pdfVIP

糖化酶降解壳聚糖的研究 ∗ 张裕卿 ,李滨县,张红柳,梁江华 （天津大学化工学院，天津 300072，中国） 摘 要：研究了糖化酶降解高分子量壳聚糖制备低分子量、高水溶性和高生物活性低聚壳聚糖的过程。通过 测定反应体系还原端基含量和粘度，探讨了温度、pH值、酶底比、时间各因素对糖化酶降解壳聚糖作用的影 响。结果表明最适宜的催化降解条件为：温度 60℃，pH值约 5.4，酶底比 0.4（V/W），时间 3.5h-4.0h。糖 化酶以内切作用方式催化壳聚糖糖苷键的断裂，并且反应符合简单的一级反应动力学和米氏酶的特性，拟合 Michaelis-Menten方程得到米氏常数 -2 K 为2.332mg/ml、V 为3.68×10 mg/min·ml。 m max 关键词：壳聚糖；糖化酶；低聚壳聚糖；降解 Study on Degradation of Chitosan by Glucoamylase ZHANG Yu-qing, LI Bin-xian, ZHANG Hong-liu, LIANG Jiang-hua (School of Chemical Engineering and Technology, Tianjin University, Tianjin 300072, China) Abstract: Chitooligosaccharides which have low molecular weight, high solubility and high physiological activities were obtained through hydrolyzed chitosan by glucoamylase. The effect of reaction conditions including temperature, pH value, time and the ratio of enzyme to substrate on the degradation of chitosan was analyzed through determined the concentration of reducing sugar and the viscosity of solution. Experimental results demonstrate the optimal temperature, pH value, time and the ratio of enzyme to substrate are 60℃, 5.4, 3.5-4.0h and 0.4(V/W). The enzymatic degradation was endo-action and conformed to simple first order kinetic. Michaelis-Menten kinetics parameters were K 2.332mg/ml and V m max 3.68×10-2mg/minml which were estimated from Lineweaver-Burk plot by mensurated the concentration of reducing sugar. Keywords: chitosan; glucoamylase; chitooligosaccharide; degradation 收稿日期：2006-08-20 作者简介：张裕卿，男，1963 年出生，生物化工专业博士